Mouse Vitamin DBP ELISA Kit | GC elisa kit

Mouse Vitamin DBP PicoKine ELISA Kit

Cat. Num. AAA1752668

• Gene Names: GC; DBP; VDB; GRD3; VDBG; VDBP; GcMAF; DBP/GC; Gc-MAF; DBP-maf; HEL-S-51
• Reactivity: Mouse
• Synonyms: Vitamin DBP; Mouse Vitamin DBP PicoKine ELISA Kit; Mouse Vitamin DBP; GC; vitamin D binding protein; Vitamin D-binding protein; DBP; VDB; Gc protein-derived macrophage activating factor; Gc-MAF; GcMAF; Gc-globulin; Group-specific component; Gc; Vitamin D-binding protein-macrophage activating factor; DBP-maf; GC elisa kit

• Reactivity: Mouse
• Specificity: Natural and recombinant Mouse GC
• Sequence Length: 493

• Samples: Cell culture supernates, lysates, serum, plasma (heparin) and urine
• Detection Range: 156pg/ml-10000pg/ml
• Sensitivity: <50pg/ml
• Intra-assay Precision: Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested on one plate to assess intra-assay precision.
• Inter-assay Precision: Inter-Assay Precision (Precision across assays):Three samples of known concentration were tested in separate assays to assess inter-assay precision.
• Preparation and Storage: Store at 4 degree C for 6 months. Store at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for GC elisa kit

Principle of the Assay: The Picokine™ Mouse GC Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Mouse GC with a 96-well strip plate that is pre-coated with antibody specific for GC. The detection antibody is a biotinylated antibody specific for GC. The capture antibody is a monoclonal antibody from rat, the detection antibody is a biotinylated polyclonal antibody from goat. The kit contains recombinant Mouse GC with immunogen: Expression system for standard: NSO, Immunogen sequence: L17-S476. The kit is analytically validated with ready to use reagents. To measure Mouse GC, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The density of the yellow product is linearly proportional to Mouse GC in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Mouse GC in the sample.!!Background/Introduction: Vitamin D-binding protein (DBP), also/originally known as gc-globulin (group-specific component), is a protein that in humans is encoded by the GC gene. It is mapped to 5 E1; 5 44.32 cM. The protein encoded by this gene belongs to the albumin gene family. It is a multifunctional protein found in plasma, ascitic fluid, cerebrospinal fluid and on the surface of many cell types. It binds to vitamin D and its plasma metabolites and transports them to target tissues. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

NCBI and Uniprot Product Information

• NCBI GI #: 324021745
• NCBI GeneID: 2638
• NCBI Accession #: NP_001191236.1
• NCBI GenBank Nucleotide #: NP_001191236.1
• UniProt Accession #: P02774
• NCBI Official Full Name: vitamin D-binding protein isoform 3
• NCBI Official Synonym Full Names: GC vitamin D binding protein
• NCBI Official Symbol: GC
• NCBI Official Synonym Symbols: DBP; VDB; GRD3; VDBG; VDBP; GcMAF; DBP/GC; Gc-MAF; DBP-maf; HEL-S-51
• NCBI Protein Information: vitamin D-binding protein
• UniProt Protein Name: Vitamin D-binding protein
• Protein Family: Vitamin D-binding protein
• UniProt Gene Name: GC
• UniProt Synonym Gene Names: DBP; VDB
• UniProt Entry Name: VTDB_HUMAN

NCBI Description

The protein encoded by this gene belongs to the albumin gene family. It is a multifunctional protein found in plasma, ascitic fluid, cerebrospinal fluid and on the surface of many cell types. It binds to vitamin D and its plasma metabolites and transports them to target tissues. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Feb 2011]

Uniprot Description

GC: Multifunctional protein found in plasma, ascitic fluid, cerebrospinal fluid, and urine and on the surface of many cell types. In plasma, it carries the vitamin D sterols and prevents polymerization of actin by binding its monomers. DBP associates with membrane-bound immunoglobulin on the surface of B-lymphocytes and with IgG Fc receptor on the membranes of T-lymphocytes. Belongs to the ALB/AFP/VDB family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 4q12-q13

Cellular Component: extracellular space; lysosomal lumen; extracellular region; cytosol

Molecular Function: vitamin D binding; actin binding; vitamin transporter activity

Biological Process: steroid metabolic process; vitamin transport; vitamin D metabolic process

Disease: Graves Disease, Susceptibility To, 1