Human gastrin ELISA Kit | GAST elisa kit

Human Gastrin ELISA Kit

Cat. Num. AAA702367

• Gene Names: GAST; GAS
• Reactivity: Human
• Synonyms: gastrin; Human Gastrin ELISA Kit; GAS; ; GAST elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of mouse GAD1. No significant cross-reactivity or interference between mouse GAD1 and analogues was observed.
• Sequence Length: 101

• Samples: Serum, Plasma, Tissue Homogenates, Cell Lysates
• Assay Type: Quantitative Sandwich
• Detection Range: 6.25 pg/ml-400 pg/ml
• Sensitivity: 1.56 pg/ml.
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for GAST elisa kit

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for GAD1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any GAD1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for GAD1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of GAD1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 4503923
• NCBI GeneID: 2520
• NCBI Accession #: NP_000796.1
• NCBI GenBank Nucleotide #: NM_000805.4
• UniProt Accession #: P01350; P78463; P78464; AAB19304
• Molecular Weight: 11,394 Da
• NCBI Official Full Name: gastrin preproprotein
• NCBI Official Synonym Full Names: gastrin
• NCBI Official Symbol: GAST
• NCBI Official Synonym Symbols: GAS
• NCBI Protein Information: gastrin; preprogastrin
• UniProt Protein Name: Gastrin
• Protein Family: Gastrin
• UniProt Gene Name: GAST
• UniProt Synonym Gene Names: GAS; G52; G34; G17; G14; G6
• UniProt Entry Name: GAST_HUMAN

NCBI Description

Gastrin is a hormone whose main function is to stimulate secretion of hydrochloric acid by the gastric mucosa, which results in gastrin formation inhibition. This hormone also acts as a mitogenic factor for gastrointestinal epithelial cells. Gastrin has two biologically active peptide forms, G34 and G17. [provided by RefSeq, Jul 2008]

Uniprot Description

Function: Gastrin stimulates the stomach mucosa to produce and secrete hydrochloric acid and the pancreas to secrete its digestive enzymes. It also stimulates smooth muscle contraction and increases blood circulation and water secretion in the stomach and intestine.