Rat Growth Associated Protein 43 ELISA Kit | GAP43 elisa kit

Rat Growth Associated Protein 43 ELISA Kit

Cat. Num. AAA723441

• Gene Names: GAP43; B-50; PP46
• Reactivity: Rat
• Synonyms: Growth Associated Protein 43; Rat Growth Associated Protein 43 ELISA Kit; GAP43 elisa kit

• Reactivity: Rat

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Sandwich
• Detection Range: 0.5-10 ng/mL
• Sensitivity: 0.1ng/ml
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Standard Curve/Testing Data

Related Product Information for GAP43 elisa kit

Intended Uses: This GAP43 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat GAP43. This ELISA kit for research use only!

Principle of the Assay: GAP43 ELISA kit applies the competitive enzyme immunoassay technique utilizing a Polyclonal anti-GAP43 antibody and an GAP43-HRP conjugate. The assay sample and buffer are incubated together with GAP43-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the GAP43 concentration since GAP43 from samples and GAP43-HRP conjugate compete for the anti-GAP43 antibody binding site. Since the number of sites is limited, as more sites are occupied by GAP43 from the sample, fewer sites are left to bind GAP43-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GAP43 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for GAP43 elisa kit

Neurobiology

NCBI and Uniprot Product Information

• NCBI GI #: 119600000
• NCBI GeneID: 2596
• Molecular Weight: 28,766 Da
• NCBI Official Full Name: growth associated protein 43
• NCBI Official Synonym Full Names: growth associated protein 43
• NCBI Official Symbol: GAP43
• NCBI Official Synonym Symbols: B-50; PP46
• NCBI Protein Information: neuromodulin; protein F1; neural phosphoprotein B-50; growth-associated protein 43; axonal membrane protein GAP-43; calmodulin-binding protein P-57; nerve growth-related peptide GAP43; neuron growth-associated protein 43
• UniProt Protein Name: Neuromodulin
• Protein Family: Neuromodulin
• UniProt Gene Name: GAP43
• UniProt Entry Name: NEUM_HUMAN

NCBI Description

The protein encoded by this gene has been termed a 'growth' or 'plasticity' protein because it is expressed at high levels in neuronal growth cones during development and axonal regeneration. This protein is considered a crucial component of an effective regenerative response in the nervous system. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

GAP43: This protein is associated with nerve growth. It is a major component of the motile growth cones that form the tips of elongating axons. Plays a role in axonal and dendritic filopodia induction. Identified in a complex containing FGFR4, NCAM1, CDH2, PLCG1, FRS2, SRC, SHC1, GAP43 and CTTN. Binds calmodulin with a greater affinity in the absence of Ca(2+) than in its presence. Belongs to the neuromodulin family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell surface

Chromosomal Location of Human Ortholog: 3q13.31

Cellular Component: filopodium membrane; axon; cytoplasm; postsynaptic density; plasma membrane; cell junction

Molecular Function: calmodulin binding; protein binding

Biological Process: cell fate commitment; glial cell differentiation; tissue regeneration; regulation of growth; axon choice point recognition; protein kinase C activation; response to wounding; regulation of filopodium formation