GALNT2 / GalNAc-T2 recombinant protein

Recombinant Human GALNT2 / GalNAc-T2 Protein (His tag)

Cat. Num. AAA2545249

• Gene Names: GALNT2; GalNAc-T2
• Purity: (94.8+3.9) % as determined by SDS-PAGE
• Synonyms: GALNT2 / GalNAc-T2; Recombinant Human GALNT2 / GalNAc-T2 Protein (His tag); GALNT2; GALNT2 / GalNAc-T2 recombinant protein

• Host: Human Cells
• Purity/Purification: (94.8+3.9) % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 25 mM Tris, 150 mM NaCl, 0.05% Brij-35, pH 7.5
• Sequence Length: 571

• Application Notes: The recombinant human GALNT2 comprises 540 amino acids and has a predicted molecular mass of 61.6 kDa. The apparent molecular mass of the protein is approximately 57-65 and 46 kDa in SDS-PAGE under reducing conditions.
• Predicted N Terminal: His
• Endotoxin: < 1.0 EU per mug of the protein as determined by the LAL method
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for GALNT2 / GalNAc-T2 recombinant protein

Background: GALNT2, also known as GalNAc-T2, is a member of the GalNAc-transferases family. Members of this family transfer an N-acetyl galactosamine to the hydroxyl group of a serine or threonine residue in the first step of O-linked oligosaccharide biosynthesis. GALNT2 may play a role in lipid metabolism. It catalyzes the initial reaction in O-linked oligosaccharide biosynthesis, the transfer of an N-acetyl-D-galactosamine residue to a serine or threonine residue on the protein receptor. GALNT2 has a broad spectrum of substrates for peptides such as EA2, Muc5AC, Muc1a, Muc1b.

Description: A DNA sequence encoding the human GALNT2 (NP_004472.1) (Lys52-Gln571) was expressed with an N-terminal polyhistidine tag.

NCBI and Uniprot Product Information

• NCBI GI #: 4758412
• NCBI GeneID: 2590
• NCBI Accession #: NP_004472.1
• NCBI GenBank Nucleotide #: NM_004481.4
• UniProt Accession #: Q10471; Q9NPY4; A8K1Y3; B7Z8V8; C5HU00
• Molecular Weight: 30,256 Da
• NCBI Official Full Name: polypeptide N-acetylgalactosaminyltransferase 2
• NCBI Official Synonym Full Names: polypeptide N-acetylgalactosaminyltransferase 2
• NCBI Official Symbol: GALNT2
• NCBI Official Synonym Symbols: GalNAc-T2
• NCBI Protein Information: polypeptide N-acetylgalactosaminyltransferase 2
• UniProt Protein Name: Polypeptide N-acetylgalactosaminyltransferase 2
• UniProt Gene Name: GALNT2
• UniProt Synonym Gene Names: GalNAc-T2; pp-GaNTase 2

NCBI Description

This gene encodes a member of the glycosyltransferase 2 protein family. Members of this family initiate mucin-type O-glycoslation of peptides in the Golgi apparatus. The encoded protein may be involved in O-linked glycosylation of the immunoglobulin A1 hinge region. This gene may influence triglyceride levels, and may be involved Type 2 diabetes, as well as several types of cancer. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2014]

Uniprot Description

GALNT2: an enzyme that catalyzes the initial reaction in O-linked oligosaccharide biosynthesis, the transfer of an N-acetyl-D-galactosamine residue to a serine or threonine residue on the protein receptor. A single-pass type II membrane protein that localizes to the Golgi apparatus membrane. Has a broad spectrum of substrates for peptides such as EA2, Muc5AC, Muc1a, Muc1b.

Protein type: EC 2.4.1.41; Glycan Metabolism - O-glycan biosynthesis; Membrane protein, integral; Transferase

Chromosomal Location of Human Ortholog: 1q42.13

Cellular Component: endoplasmic reticulum membrane; Golgi apparatus; Golgi membrane; Golgi stack; membrane; perinuclear region of cytoplasm

Molecular Function: manganese ion binding; polypeptide N-acetylgalactosaminyltransferase activity; protein binding

Biological Process: immunoglobulin biosynthetic process; O-glycan processing; protein amino acid O-linked glycosylation; protein amino acid O-linked glycosylation via serine; protein amino acid O-linked glycosylation via threonine