Human galanin prepropeptide ELISA Kit | GAL elisa kit

Human galanin, GAL ELISA Kit

Cat. Num. AAA703354

• Gene Names: GAL; GALN; GLNN; GMAP; GAL-GMAP
• Reactivity: Human
• Synonyms: galanin prepropeptide; Human galanin; GAL ELISA Kit; GALN; GLNN; GMAP; MGC40167; galanin; galanin-message-associated peptide; galanin-related peptide; GAL elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Human GAL. No significant cross-reactivity or interference between Human GAL and analogues was observed.
• Sequence Length: 123

• Samples: Serum, plasma, tissue homogenates
• Detection Range: 4.7 pg/ml-300 pg/ml
• Sensitivity: 1.17 pg/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%; Three samples of known concentration were tested twenty times on one plate to assess.; Inter-assay Precision (Precision between assays): CV% is less than 10%; Three samples of known concentration were tested in twenty assays to assess.
• Detection Wavelength: 450 nm
• Sample Volume: 50-100ul
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for GAL elisa kit

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to GAL. Standards or samples are then added to the appropriate microtiter plate wells with a biotin conjugated antibody preparation specific for GAL and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain GAL, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of GAL in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 27804313
• NCBI GeneID: 51083
• NCBI Accession #: NP_057057.2
• NCBI GenBank Nucleotide #: NM_015973.3
• UniProt Accession #: P22466; Q14413
• Molecular Weight: 13,302 Da
• NCBI Official Full Name: galanin peptides preproprotein
• NCBI Official Synonym Full Names: galanin/GMAP prepropeptide
• NCBI Official Symbol: GAL
• NCBI Official Synonym Symbols: GALN; GLNN; GMAP; GAL-GMAP
• NCBI Protein Information: galanin peptides; galanin prepropeptide; galanin-related peptide; galanin-message-associated peptide
• UniProt Protein Name: Galanin peptides
• Protein Family: Galanin
• UniProt Gene Name: GAL
• UniProt Synonym Gene Names: GAL1; GALN; GLNN; GMAP
• UniProt Entry Name: GALA_HUMAN

NCBI Description

Galanin is small neuropeptide that functions as a cellular messenger within the central and peripheral nervous systems, modulating diverse physiologic functions (Mechenthaler, 2008 [PubMed 18500643]).[supplied by OMIM, Jan 2009]

Uniprot Description

Function: Contracts smooth muscle of the gastrointestinal and genitourinary tract, regulates growth hormone release, modulates insulin release, and may be involved in the control of adrenal secretion.