Mouse Growth arrest and DNA damage-inducible protein GADD45 gamma ELISA Kit | Gadd45g elisa kit

Mouse Growth arrest and DNA damage-inducible protein GADD45 gamma ELISA Kit

Cat. Num. AAA764413

• Gene Names: GADD45G; CR6; DDIT2; GRP17; GADD45gamma
• Reactivity: Mouse
• Synonyms: Growth arrest and DNA damage-inducible protein GADD45 gamma; Mouse Growth arrest and DNA damage-inducible protein GADD45 gamma ELISA Kit; Gadd45g/Cr6/Growth arrest and DNA damage-inducible protein GADD45 gamma/Cytokine-responsive protein CR6; Gadd45g elisa kit

• Reactivity: Mouse
• Sequence Length: 159

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 0.156-10ng/ml
• Sensitivity: 0.094ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for Gadd45g elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. anti-Mouse Gadd45g antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Mouse Gadd45g antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Mouse Gadd45g amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Mouse Gadd45g can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 5729836
• NCBI GeneID: 10912
• NCBI Accession #: NP_006696.1
• NCBI GenBank Nucleotide #: NM_006705.3
• Molecular Weight: 17,121 Da
• NCBI Official Full Name: growth arrest and DNA damage-inducible protein GADD45 gamma
• NCBI Official Synonym Full Names: growth arrest and DNA damage inducible gamma
• NCBI Official Symbol: GADD45G
• NCBI Official Synonym Symbols: CR6; DDIT2; GRP17; GADD45gamma
• NCBI Protein Information: growth arrest and DNA damage-inducible protein GADD45 gamma
• UniProt Protein Name: Growth arrest and DNA damage-inducible protein GADD45 gamma
• Protein Family: Growth arrest and DNA damage-inducible protein
• UniProt Gene Name: GADD45G
• UniProt Synonym Gene Names: CR6; DDIT2; DDIT-2
• UniProt Entry Name: GA45G_HUMAN

NCBI Description

This gene is a member of a group of genes whose transcript levels are increased following stressful growth arrest conditions and treatment with DNA-damaging agents. The protein encoded by this gene responds to environmental stresses by mediating activation of the p38/JNK pathway via MTK1/MEKK4 kinase. The GADD45G is highly expressed in placenta. [provided by RefSeq, Jul 2008]

Uniprot Description

GADD45G: Involved in the regulation of growth and apoptosis. Mediates activation of stress-responsive MTK1/MEKK4 MAPKKK. Belongs to the GADD45 family.

Protein type: Apoptosis; DNA repair, damage; Nuclear receptor co-regulator

Chromosomal Location of Human Ortholog: 9q22.1-q22.2

Cellular Component: cytoplasm; nucleus

Molecular Function: protein binding

Biological Process: activation of MAPKK activity; activation of MAPKKK activity; apoptosis; cell differentiation; multicellular organismal development; negative regulation of protein kinase activity; positive regulation of apoptosis; positive regulation of JNK cascade; regulation of cell cycle; response to stress