Rat GABA (A) receptor-associated protein like 1 ELISA Kit | GABARAPL1 elisa kit

Rat Gamma-aminobutyric acid receptor-associated protein-like 1, GABARAPL1 ELISA Kit

Cat. Num. AAA9328399

• Gene Names: Gabarapl1; Gec1
• Reactivity: Rat
• Synonyms: GABA (A) receptor-associated protein like 1; Rat Gamma-aminobutyric acid receptor-associated protein-like 1; GABARAPL1 ELISA Kit; Rat Gamma-aminobutyric acid receptor-associated protein-like 1 (GABARAPL1) ELISA kit; APG8-LIKE; APG8L; ATG8; ATG8L; GEC1; early estrogen-regulated protein; GABARAPL1 elisa kit

• Reactivity: Rat
• Specificity: No significant cross-reactivity or interference between Rat GABARAPL1 and analogues was observed.

• Samples: Serum, Plasma, Tissue Homogenate, Feces and Urine
• Assay Type: Sandwich
• Detection Range: 0.5 ng/ml - 16 ng/ml.
• Sensitivity: 0.1 ng/ml.
• Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic applications! It is intended to be determinated GABARAPL1 concentrations in Rat serum, plasma and other body fluids. Using Purified Rat GABARAPL1 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add GABARAPL1 and GABARAPL1 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of GABARAPL1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• Intra-assay Precision: Intra-assay CV (%) is less than 15%.
• Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]
• Preparation and Storage: Store all reagents at 2-8 degree C

Related Product Information for GABARAPL1 elisa kit

Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only! It is intended to be determinated GABARAPL1 concentrations in Rat serum, plasma and other body fluids. Using Purified Rat GABARAPL1 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add GABARAPL1 and GABARAPL1 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of GABARAPL1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 112982886
• NCBI GeneID: 689161
• NCBI Accession #: NP_001037759.1
• NCBI GenBank Nucleotide #: NM_001044294.1
• UniProt Accession #: Q0VGK0
• Molecular Weight: 14,044 Da
• NCBI Official Full Name: gamma-aminobutyric acid receptor-associated protein-like 1
• NCBI Official Synonym Full Names: GABA(A) receptor-associated protein like 1
• NCBI Official Symbol: Gabarapl1
• NCBI Official Synonym Symbols: Gec1
• NCBI Protein Information: gamma-aminobutyric acid receptor-associated protein-like 1; GEC-1; glandular epithelial cell protein 1; GABA(A) receptor-associated protein-like 1; gamma-aminobutyric acid (GABA(A)) receptor-associated protein-like 1
• UniProt Protein Name: Gamma-aminobutyric acid receptor-associated protein-like 1
• Protein Family: Gamma-aminobutyric acid receptor-associated protein
• UniProt Gene Name: Gabarapl1
• UniProt Synonym Gene Names: Gec1; GEC-1
• UniProt Entry Name: GBRL1_RAT

Uniprot Description

GABARAPL1: is a ubiquitin-like protein that is a constituent of the ATG8-conjugation system, one of two evolutionarily conserved phosphatidylethanolamine conjugation systems necessary for the formation of the autophagosome. The human ATG8 system includes seven ubiquitin-like light chain proteins (LCPs) that are homologs of yeast LC3: MAP1LC3A, -B, -C, GABARAP, GABARAPL1, -2, and -3. Pro-LCPs are cleaved by ATG4B to expose a C-terminal glycine residue, the cytosolic LCP-I form. The exposed C-terminus is conjugated to the head group amine of phosphatidylethanolamine (PE) through an amide bond by a sequence of ubiquitination-like reactions that involves an E1 (ATG7), an E2 (ATG3), and an E3 (a complex including ATG5, ATG12, and ATG16L). The PE-congugated form (LCP-II) is tightly associated with the autophagosomal membrane. The LCP-II forms can also be delipidated by the ATG4 proteases: most of the LCPs are delipidated and liberated from the membrane before autophagosomes fuse with lysosomes. Increases cell-surface expression of kappa-type opioid receptor through facilitating anterograde intracellular trafficking of the receptor. Belongs to the MAP1 LC3 family. Interacts with GABRG2 and beta-tubulin. Interacts with KOR-1.

Protein type: Ubiquitin-like modifier; Vesicle; Autophagy; Microtubule-binding; Adaptor/scaffold