Typical Testing Data/Standard Curve (for reference only)
Chicken COL7 ELISA Kit | COL7 elisa kit
Chicken COL7 (Collagen Type VII) ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to COL7. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for COL7 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain COL7, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of COL7. You can calculate the concentration of COL7 in the samples by comparing the OD of the samples to the standard curve.
NCBI and Uniprot Product Information
Uniprot Description
COL7A1: the alpha chain of type VII collagen, an extra-cellular basement membrane protein restricted to the zone beneath stratified squamous epithelia. Type VII collagen fibrils are composed of three identical alpha collagen chains. Forms anchoring fibrils, which may contribute to epithelial basement membrane organization and adherence by interacting with extracellular matrix (ECM) proteins such as type IV collagen. Dystrophic epidermolysis bullosa can result from defects in this protein or an autoimmune response made to type VII collagen. Homotrimer. Interacts with MIA3, facilitating its loading into transport carriers and subsequent secretion. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Inhibitor; Secreted; Secreted, signal peptide; Extracellular matrix
Cellular Component: proteinaceous extracellular matrix; extracellular space; collagen; extracellular region; basement membrane
Molecular Function: serine-type endopeptidase inhibitor activity; identical protein binding; protease inhibitor activity
Biological Process: negative regulation of peptidase activity; cell adhesion
Research Articles on COL7
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Product Notes
The Chicken COL7 col7a1 (Catalog #AAA2500918) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2500918 ELISA Kit recognizes Chicken COL7. It is sometimes possible for the material contained within the vial of "COL7, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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