GRB2 Associated Binding Protein 1 (GAB1) Recombinant Protein | GAB1 recombinant protein

Recombinant GRB2 Associated Binding Protein 1 (GAB1)

Cat. Num. AAA2018508

• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: GRB2 Associated Binding Protein 1 (GAB1); Recombinant GRB2 Associated Binding Protein 1 (GAB1); GAB1 recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.; MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- NKLRKDA SSQDCYDIPR AFPSDRSSSL EGFHNHFKVK NVLTVGSVSS EELDENYVPM NPNSPPRQHS SSFTEPIQEA NYVPMTPGTF DFSSFGMQVP PPAHMGFRSS PKTPPRRPVP VADCEPPPVD RNLKPDRKGQ SPKILRLKPH GLERTDSQTI GDFATRRKVK PAPLEIKPLP EWEELQAPVR SPITRSFARD SSRFPMSPRP DSVHSTTSSS DSHDSEENYV PMNPNLSSED PNLFGSNSLDGGSSPMIKPK GDKQVE
• Sequence Length: 694

• Applicable Applications for GAB1 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Homo sapiens (Human)
• Expression System: Prokaryotic expression
• Residues: Asn394~Glu656 (Accession # Q13480) with two N-terminal Tags, His-tag and T7-tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Predicted Isoelectric Point: 7.3
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 46370073
• NCBI GeneID: 2549
• NCBI Accession #: NP_002030.2
• NCBI GenBank Nucleotide #: NM_002039.3
• UniProt Accession #: Q13480; Q4W5G2; Q6P1W2; A8K152
• Molecular Weight: 33.0kDa
• NCBI Official Full Name: GRB2-associated-binding protein 1 isoform b
• NCBI Official Synonym Full Names: GRB2-associated binding protein 1
• NCBI Official Symbol: GAB1
• NCBI Protein Information: GRB2-associated-binding protein 1; GRB2-associated binder 1; growth factor receptor bound protein 2-associated protein 1
• UniProt Protein Name: GRB2-associated-binding protein 1
• Protein Family: GPI transamidase component
• UniProt Gene Name: GAB1
• UniProt Entry Name: GAB1_HUMAN

NCBI Description

The protein encoded by this gene is a member of the IRS1-like multisubstrate docking protein family. It is an important mediator of branching tubulogenesis and plays a central role in cellular growth response, transformation and apoptosis. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2008]

Uniprot Description

GAB1: Adapter protein that plays a role in intracellular signaling cascades triggered by activated receptor-type kinases. Plays a role in FGFR1 signaling. Probably involved in signaling by the epidermal growth factor receptor (EGFR) and the insulin receptor (INSR). Interacts with GRB2 and with other SH2-containing proteins. Interacts with phosphorylated LAT2. Interacts with PTPRJ. Identified in a complex containing FRS2, GRB2, GAB1, PIK3R1 and SOS1. Interacts (phosphorylated) with PTPN11. Interacts with HCK. Belongs to the GAB family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; Adaptor/scaffold

Chromosomal Location of Human Ortholog: 4q31.21

Cellular Component: cytosol

Molecular Function: protein binding; signal transducer activity; SH3/SH2 adaptor activity

Biological Process: epidermal growth factor receptor signaling pathway; cell proliferation; epidermis development; phosphoinositide-mediated signaling; fibroblast growth factor receptor signaling pathway; nerve growth factor receptor signaling pathway; heart development; insulin receptor signaling pathway; innate immune response; platelet-derived growth factor receptor signaling pathway; response to oxidative stress; regulation of cell migration; activation of JNK activity