Rabbit BAD Polyclonal Antibody | anti-Bad antibody

Mouse BAD Antibody (Center S134)

Cat. Num. AAA9217110

• Gene Names: Bad; Bbc2; AI325008
• Reactivity: Mouse; Predicted Reactivity: Rat
• Applications: Western Blot, ELISA
• Synonyms: BAD; Polyclonal Antibody; Mouse BAD Antibody (Center S134); Bcl2-associated agonist of cell death; Bcl-2-binding component 6; Bcl-xL/Bcl-2-associated death promoter; Bcl2 antagonist of cell death; Bad; Bbc6; anti-Bad antibody

• Host: Rabbit
• Reactivity: Mouse; Predicted Reactivity: Rat
• Clonality: Polyclonal
• Isotype: Ig
• Specificity: This Mouse BAD antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 112-141 amino acids from the Central region of mouse BAD.
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Sequence Length: 204

• Applicable Applications for anti-Bad antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB:~~1:1000
• Subtitle: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Cellular Location: Mitochondrion outer membrane. Cytoplasm. Note=Upon phosphorylation, locates to the cytoplasm.
• Antigen Region: 112-141 aa
• Antigen Type: Synthetic Peptide
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(All lanes : Anti-mouse BAD Antibody at 1:1000 dilutionLane 1: A20 whole cell lysateLane 2: C2C12 whole cell lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 22 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Western Blot (WB)

(mouse BAD Antibody (S134) western blot analysis in mouse testis tissue lysates (35ug/lane).This demonstrates the BAD antibody detected the BAD protein (arrow).)

Related Product Information for anti-Bad antibody

Description: BAD promotes cell death. Successfully competes for the binding to Bcl-X(L), Bcl-2 and Bcl-W, thereby affecting the level of heterodimerization of these proteins with BAX. Can reverse the death repressor activity of Bcl-X(L), but not that of Bcl-2. Appears to act as a link between growth factor receptor signaling and the apoptotic pathways.

Background: Promotes cell death. Successfully competes for the binding to Bcl-X(L), Bcl-2 and Bcl-W, thereby affecting the level of heterodimerization of these proteins with BAX. Can reverse the death repressor activity of Bcl-X(L), but not that of Bcl-2. Appears to act as a link between growth factor receptor signaling and the apoptotic pathways.

Product Categories/Family for anti-Bad antibody

Primary Antibodies; Antibody Collections; Cancer; Cell Biology; Signal Transduction; Amyotrophic Lateral Sclerosis (ALS)

References

Santidrian, A.F., et al. Blood 116 (16):3023-3032 (2010). Frenzel, A., et al. Blood 115 (5):995-1005 (2010). Quoyer, J., et al. J. Biol. Chem. 285 (3):1989-2002 (2010). Polzien, L., et al. J. Biol. Chem. 284 (41):28004-28020 (2009). Wu, X., et al. Diabetologia 52 (10):2130-2141 (2009).

NCBI and Uniprot Product Information

• NCBI GI #: 6671610
• NCBI GeneID: 12015
• NCBI Accession #: NP_031548.1
• NCBI GenBank Nucleotide #: NM_007522.3
• UniProt Accession #: Q61337; O35147
• Molecular Weight: 22080 Da
• NCBI Official Full Name: bcl2-associated agonist of cell death isoform 1
• NCBI Official Synonym Full Names: BCL2-associated agonist of cell death
• NCBI Official Symbol: Bad
• NCBI Official Synonym Symbols: Bbc2; AI325008
• NCBI Protein Information: bcl2-associated agonist of cell death
• UniProt Protein Name: Bcl2-associated agonist of cell death
• Protein Family: Bcl2-associated agonist of cell death
• UniProt Gene Name: Bad
• UniProt Synonym Gene Names: Bbc6; BAD
• UniProt Entry Name: BAD_MOUSE

Uniprot Description

BAD: a proapoptotic member of the Bcl-2 family. Displaces Bax from binding to Bcl-2 and Bcl-xL, resulting in cell death. Survival factors such as IL-3 can inhibit the apoptotic activity of Bad inducing the phosphorylation of Bad by Akt and p90RSK. 14-3-3 proteins bind phosphorylated Bad, inhibiting its binding to Bcl-2 and Bcl-xL. Phosphorylation by mitochondria-anchored PKA in the BH3 domain can block the dimerization of Bad and Bcl-xL.

Protein type: Apoptosis

Cellular Component: cytoplasm; cytosol; membrane; mitochondrial outer membrane; mitochondrion

Molecular Function: caspase activator activity; lipid binding; phospholipid binding; protein binding; protein heterodimerization activity; protein kinase B binding; protein kinase binding; protein phosphatase 2B binding; protein phosphatase binding

Biological Process: ADP metabolic process; apoptosis; ATP metabolic process; caspase activation; cell proliferation; cellular process regulating host cell cycle in response to virus; cytokine and chemokine mediated signaling pathway; DNA damage response, signal transduction resulting in induction of apoptosis; glucose catabolic process; glucose homeostasis; induction of apoptosis via death domain receptors; negative regulation of cytolysis; pore complex biogenesis; positive regulation of apoptosis; positive regulation of apoptosis by virus; positive regulation of B cell differentiation; positive regulation of caspase activity; positive regulation of epithelial cell proliferation; positive regulation of glucokinase activity; positive regulation of insulin secretion; positive regulation of proteolysis; positive regulation of T cell differentiation; regulation of apoptosis; regulation of caspase activity; regulation of mitochondrial membrane permeability; release of cytochrome c from mitochondria; suppression by virus of host apoptosis