Hamster Gibberellic Acid ELISA Kit | GA elisa kit
Hamster Gibberellic Acid ELISA Kit
NCBI and Uniprot Product Information
Uniprot Description
Function: Methylates the carboxyl group of several gibberellins (GAs). Substrate preference is GA4 > GA34 > GA9 > GA3 > GA1 > GA51 > GA20. No activity with diterpenes abietic acid and ent-kaurenoic acid. Ref.5
Catalytic activity: S-adenosyl-L-methionine + gibberellin A4 = S-adenosyl-L-homocysteine + methyl gibberellin A4. Ref.5
Cofactor: Binds 1 magnesium ion per subunit
By similarity.
Enzyme regulation: Down-regulated by Zn2+, Cu2+ and Fe3+. No effect of K+, NH4+, Na+, Ca2+, Mg2+, Mn2+ and Fe2+. Ref.5
Tissue specificity: Expressed in siliques and germinating seeds. Not detected in leaves, stems, flowers and roots. Ref.5
Developmental stage: Expression begins at early stages of silique development, peaks in the second half of this process and decreases after the start of desiccation. Ref.5
Induction: Up-regulated by alamethicin, but not by herbivory. Ref.4 Ref.5
Disruption phenotype: No visible phenotype, even in gamt1 and gamt2 double mutants. Ref.5
Miscellaneous: Overexpression of GAMT2 results in dwarf phenotype (Ref.5).
Sequence similarities: Belongs to the methyltransferase superfamily. Type-7 methyltransferase family. SABATH subfamily.
Biophysicochemical propertiesKinetic parameters:kcat is 0.0015 sec(-1) for GA4. kcat is 0.0018 sec(-1) for GA9.KM=5.9 µM for GA4 Ref.5KM=1.9 µM for GA9pH dependence:Optimum pH is 8.0.
Sequence caution: The sequence BAB11257.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.
Research Articles on GA
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Product Notes
The Hamster GA gamt2 (Catalog #AAA050001) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA050001 ELISA Kit recognizes Hamster GA. It is sometimes possible for the material contained within the vial of "Gibberellic Acid, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
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