Rabbit anti-Human, Mouse DDX4 Polyclonal Antibody | anti-DDX4 antibody

DDX4, NT (DDX4, VASA, Probable ATP-dependent RNA helicase DDX4, DEAD box protein 4, Vasa homolog) (PE)

Cat. Num. AAA6291474

• Gene Names: DDX4; VASA
• Reactivity: Human, Mouse
• Applications: Western Blot
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: DDX4; Polyclonal Antibody; NT (DDX4; VASA; Probable ATP-dependent RNA helicase DDX4; DEAD box protein 4; Vasa homolog) (PE); Vasa homolog; anti-DDX4 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human DDX4. Species Crossreactivity: mouse
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-DDX4 antibody: Western Blot (WB), FLISA
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 103-131 in the N-terminal region of human DDX4. Species sequence homology: monkey
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in WiDr cell line lysates (35ug/lane) usingMBS644176. This demonstrates the MBS644176 detected the DDX4 protein (arrow).)

Western Blot (WB)

(Western Blot analysis in mouse testis tissue lysates (35ug/lane) usingMBS644176. This demonstrates the MBS644176 detected the DDX4 protein (arrow).)

Related Product Information for anti-DDX4 antibody

DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which is a homolog of VASA proteins in Drosophila and several other species. The gene is specifically expressed in the germ cell lineage in both sexes and functions in germ cell development. Multiple transcript variants encoding different isoforms have been found for this gene.

Product Categories/Family for anti-DDX4 antibody

Antibodies; DEAD Box Proteins

References

1. Tilgner, K., et al. Stem Cells 28(1):84-92(2010). 2. Sugimoto, K., et al. J. Hum. Genet. 54(8):450-456(2009). 3. Hashimoto, H., et al. Gynecol. Oncol. 111(2):312-319(2008). 4. Albamonte, M.S., et al. Hum. Reprod. 23(8):1895-1901(2008). 5. Guo, X., et al. Asian J. Androl. 9(3):339-344(2007).

NCBI and Uniprot Product Information

• NCBI GI #: 262231852
• NCBI GeneID: 54514
• NCBI Accession #: NP_001160005.1; NP_001136021.1
• NCBI GenBank Nucleotide #: NM_001166533.1
• UniProt Accession #: Q9NQI0; Q5M7Z3; Q86VX0; Q9NT92; Q9NYB1; A8K8Q2; B3KSF4; D6RDK4; E9PCD8
• Molecular Weight: 77,056 Da
• NCBI Official Full Name: probable ATP-dependent RNA helicase DDX4 isoform 3
• NCBI Official Synonym Full Names: DEAD (Asp-Glu-Ala-Asp) box polypeptide 4
• NCBI Official Symbol: DDX4
• NCBI Official Synonym Symbols: VASA
• NCBI Protein Information: probable ATP-dependent RNA helicase DDX4; vasa homolog; DEAD box protein 4; DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 4
• UniProt Protein Name: Probable ATP-dependent RNA helicase DDX4
• Protein Family: Probable ATP-dependent RNA helicase
• UniProt Gene Name: DDX4
• UniProt Synonym Gene Names: VASA
• UniProt Entry Name: DDX4_HUMAN

NCBI Description

DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which is a homolog of VASA proteins in Drosophila and several other species. The gene is specifically expressed in the germ cell lineage in both sexes and functions in germ cell development. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2009]