Typical Testing Data/Standard Curve (for reference only)
Canine Granulocyte colony-stimulating factor (CSF3) ELISA Kit | CSF3 elisa kit
Canine Granulocyte colony-stimulating factor (CSF3) ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: G-CSF ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-G-CSF antibody and an G-CSF-HRP conjugate. The assay sample and buffer are incubated together with G-CSF-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the G-CSF concentration since G-CSF from samples and G-CSF-HRP conjugate compete for the anti-G-CSF antibody binding site. Since the number of sites is limited, as more sites are occupied by G-CSF from the sample, fewer sites are left to bind G-CSF-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The G-CSF concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a cytokine that controls the production, differentiation, and function of granulocytes. The active protein is found extracellularly. Alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, May 2010]
Uniprot Description
G-CSF: Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. This CSF induces granulocytes. Belongs to the IL-6 superfamily. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Cytokine; Cell cycle regulation; Secreted, signal peptide; Secreted
Chromosomal Location of Human Ortholog: 17q11.2-q12
Cellular Component: extracellular space
Molecular Function: enzyme binding; growth factor activity; cytokine activity; granulocyte colony-stimulating factor receptor binding
Biological Process: positive regulation of myeloid cell differentiation; positive regulation of protein binding; granulocyte differentiation; cytokine and chemokine mediated signaling pathway; multicellular organismal development; positive regulation of peptidyl-serine phosphorylation; positive regulation of phosphoinositide 3-kinase cascade; positive regulation of protein kinase B signaling cascade; positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of actin filament polymerization; positive regulation of cell proliferation; positive regulation of transcription factor import into nucleus; positive regulation of transcription from RNA polymerase II promoter; immune response
Research Articles on CSF3
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Product Notes
The Canine CSF3 csf3 (Catalog #AAA7250330) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7250330 ELISA Kit recognizes Canine CSF3. It is sometimes possible for the material contained within the vial of "Granulocyte colony-stimulating factor (CSF3), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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