FTO / Alpha-Ketoglutarate-Dependent Dioxygenase FTO Recombinant Protein | FTO recombinant protein
Human FTO / Alpha-Ketoglutarate-Dependent Dioxygenase FTO Recombinant Protein
Gene Names
FTO; GDFD; ALKBH9; BMIQ14
Applications
ELISA, Western Blot
Purity
>95% by SDS-PAGE
Synonyms
FTO / Alpha-Ketoglutarate-Dependent Dioxygenase FTO; Human FTO / Alpha-Ketoglutarate-Dependent Dioxygenase FTO Recombinant Protein; Alpha-ketoglutarate-dependent dioxygenase FTO; Fat mass and obesity-associated protein; KIAA1752; FTO recombinant protein
Host
E Coli
Purity/Purification
>95% by SDS-PAGE
Form/Format
Liquid; 50mM NaH2PO4, 500mM NaCl Buffer with 500mM Imidazole, 10% Glycerol (PH8.0)
Concentration
Reconstitution Dependent (varies by lot)
Sequence Length
505
Applicable Applications for FTO recombinant protein
ELISA (EIA), Western Blot (WB), Antigen Protein (AP), Protein Array
Species
Human
Tag
His
Subunit
Monomer. May also exist as homodimer.
Entry Function
Dioxygenase that repairs alkylated DNA and RNA by oxidative demethylation. Has highest activity towards single-stranded RNA containing 3-methyluracil, followed by single-stranded DNA containing 3-methylthymine. Has low demethylase activity towards single-stranded DNA containing 1-methyladenine or 3-methylcytosine (PubMed:18775698, PubMed:20376003). Specifically demethylates N(6)-methyladenosine (m6A) RNA, the most prevalent internal modification of messenger RNA (mRNA) in higher eukaryotes (PubMed:22002720, PubMed:26458103). Has no activity towards 1-methylguanine. Has no detectable activity towards double-stranded DNA. Requires molecular oxygen, alpha-ketoglutarate and iron. Contributes to the regulation of the global metabolic rate, energy expenditure and energy homeostasis. Contributes to the regulation of body size and body fat accumulation (PubMed:18775698, PubMed:20376003). In particular, it is involved in the regulation of thermogenesis and the control of adipocyte differentiation into brown or white fat cells (PubMed:26287746).
Preparation and Storage
Store at -20 degree C. Avoid repeated freezing and thawing.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
alpha-ketoglutarate-dependent dioxygenase FTO isoform 3
NCBI Official Synonym Full Names
FTO alpha-ketoglutarate dependent dioxygenase
NCBI Official Symbol
FTO
NCBI Official Synonym Symbols
GDFD; ALKBH9; BMIQ14
NCBI Protein Information
alpha-ketoglutarate-dependent dioxygenase FTO
UniProt Protein Name
Alpha-ketoglutarate-dependent dioxygenase FTO
Protein Family
UniProt Gene Name
FTO
UniProt Synonym Gene Names
KIAA1752
UniProt Entry Name
FTO_HUMAN
NCBI Description
This gene is a nuclear protein of the AlkB related non-haem iron and 2-oxoglutarate-dependent oxygenase superfamily but the exact physiological function of this gene is not known. Other non-heme iron enzymes function to reverse alkylated DNA and RNA damage by oxidative demethylation. Studies in mice and humans indicate a role in nervous and cardiovascular systems and a strong association with body mass index, obesity risk, and type 2 diabetes. [provided by RefSeq, Jul 2011]
Research Articles on FTO
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Product Notes
The FTO fto (Catalog #AAA2903404) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's FTO / Alpha-Ketoglutarate-Dependent Dioxygenase FTO can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Antigen Protein (AP), Protein Array. Researchers should empirically determine the suitability of the FTO fto for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "FTO / Alpha-Ketoglutarate-Dependent Dioxygenase FTO, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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