Rabbit anti-Human CD74 Polyclonal Antibody | anti-CD74 antibody

CD74 antibody

Cat. Num. AAA9402600

• Gene Names: CD74; II; DHLAG; HLADG; Ia-GAMMA
• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry
• Purity: Purified by antigen-affinity chromatography.
• Synonyms: CD74; Polyclonal Antibody; CD74 antibody; anti-CD74 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Purity/Purification: Purified by antigen-affinity chromatography.
• Form/Format: Supplied in 0.1M Tris-buffered saline with 10% Glycerol (pH7.0). 0.01% Thimerosal was added as a preservative.
• Sequence Length: 160

• Applicable Applications for anti-CD74 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Western blotting: 1:500-1:3000; Immunohistochemistry: 1:100-1:500
• Immunogen Type: Peptide
• Immunogen Description: Synthetic peptide contain a sequence corresponding to a region within amino acids 1 and 28 of CD74
• Target Name: CD74
• Preparation and Storage: Store at -20 degree C for long term preservation (recommended). Store at 4 degree C for short term use.

Testing Data

(Sample (30 ug of whole cell lysate) A: Raji 12% SDS PAGE Primary antibody diluted at 1: 5000)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded SW480 xenograft, using CD74 antibody at 1: 500 dilution.)

Product Categories/Family for anti-CD74 antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 68448537
• NCBI GeneID: 972
• NCBI Accession #: NP_001020329
• NCBI GenBank Nucleotide #: NM_001025158.2
• Molecular Weight: 18,328 Da
• NCBI Official Full Name: HLA class II histocompatibility antigen gamma chain isoform c
• NCBI Official Synonym Full Names: CD74 molecule, major histocompatibility complex, class II invariant chain
• NCBI Official Symbol: CD74
• NCBI Official Synonym Symbols: II; DHLAG; HLADG; Ia-GAMMA
• NCBI Protein Information: HLA class II histocompatibility antigen gamma chain
• UniProt Protein Name: HLA class II histocompatibility antigen gamma chain
• Protein Family: H-2 class II histocompatibility antigen
• UniProt Gene Name: CD74
• UniProt Synonym Gene Names: DHLAG; Ii
• UniProt Entry Name: HG2A_HUMAN

NCBI Description

The protein encoded by this gene associates with class II major histocompatibility complex (MHC) and is an important chaperone that regulates antigen presentation for immune response. It also serves as cell surface receptor for the cytokine macrophage migration inhibitory factor (MIF) which, when bound to the encoded protein, initiates survival pathways and cell proliferation. This protein also interacts with amyloid precursor protein (APP) and suppresses the production of amyloid beta (Abeta). Multiple alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Aug 2011]

Uniprot Description

CD74: Plays a critical role in MHC class II antigen processing by stabilizing peptide-free class II alpha/beta heterodimers in a complex soon after their synthesis and directing transport of the complex from the endoplasmic reticulum to the endosomal/lysosomal system where the antigen processing and binding of antigenic peptides to MHC class II takes place. Serves as cell surface receptor for the cytokine MIF. A chromosomal aberration involving CD74 is found in a non-small cell lung tumor. Results in the formation of a CD74- ROS1 chimeric protein. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Receptor, cytokine; Endoplasmic reticulum; Membrane protein, integral

Chromosomal Location of Human Ortholog: 5q32

Cellular Component: cell surface; external side of plasma membrane; Golgi membrane; integral to membrane; intracellular; late endosome; lysosomal lumen; lysosomal membrane; membrane; MHC class II protein complex; multivesicular body; plasma membrane; trans-Golgi network membrane; vacuole

Molecular Function: beta-amyloid binding; cytokine binding; hematopoietin/interferon-class (D200-domain) cytokine receptor activity; identical protein binding; MHC class II protein binding; MHC class II protein binding, via antigen binding groove; nitric-oxide synthase binding; protein binding

Biological Process: activation of MAPK activity; antigen processing and presentation of endogenous antigen; antigen processing and presentation of exogenous peptide antigen via MHC class II; blood coagulation; cell proliferation; chaperone cofactor-dependent protein folding; defense response; immunoglobulin mediated immune response; intracellular protein transport; leukocyte migration; negative regulation of apoptosis; negative regulation of DNA damage response, signal transduction by p53 class mediator; negative regulation of mature B cell apoptosis; negative regulation of peptide secretion; negative regulation of T cell differentiation; negative thymic T cell selection; positive regulation of B cell proliferation; positive regulation of cytokine and chemokine mediated signaling pathway; positive regulation of dendritic cell antigen processing and presentation; positive regulation of fibroblast proliferation; positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of T cell differentiation; positive regulation of T-helper 2 type immune response; positive thymic T cell selection; prostaglandin biosynthetic process; protein complex assembly; regulation of macrophage activation; signal transduction; T cell selection