Rabbit anti-Human CNO6L Polyclonal Antibody | anti-CNO6L antibody

CNO6L, CT (CNOT6L, CCR4B, CCR4-NOT transcription complex subunit 6-like, Carbon catabolite repressor protein 4 homolog B) (Azide free) (HRP)

Cat. Num. AAA6287266

• Gene Names: CNOT6L; CCR4b
• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: CNO6L; Polyclonal Antibody; CT (CNOT6L; CCR4B; CCR4-NOT transcription complex subunit 6-like; Carbon catabolite repressor protein 4 homolog B) (Azide free) (HRP); CNOT6L; Carbon catabolite repressor protein 4 homolog B; anti-CNO6L antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human CNO6L.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-CNO6L antibody: Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 414-442 in the C-terminal region of human CNO6L, UniProt Accession #Q96LI5. Species sequence homology: mouse, xenopus
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunohistochemistry (IHC)

(immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining.)

Western Blot (WB)

(034049 western blot analysis in Sk-Br-3 cell line tissue lysates (35ug/lane).This demonstrates the CNO6L antibody detected the CNO6L protein (arrow).)

Related Product Information for anti-CNO6L antibody

CNO6L plays a role in the deadenylation of mRNAs in the cytoplasm. CNO6L has 3'-5' poly(A) exoribonuclease activity for synthetic poly(A) RNA substrate. CNO6L may be involved in the deadenylation-dependent degradation of mRNAs through the 3'-UTR AU-rich element-mediated mechanism. This protein involved in deadenylation-dependent degradation of CDKN1B mRNA.

Product Categories/Family for anti-CNO6L antibody

Antibodies; Transcription Factors

References

Miyasaka, T., et al. Cancer Sci. 99(4):755-761(2008). Morita, M., et al. Mol. Cell. Biol. 27(13):4980-4990(2007). Ewing, R.M., et al. Mol. Syst. Biol. 3, 89 (2007).

NCBI and Uniprot Product Information

• NCBI GI #: 166216089
• NCBI GeneID: 246175
• UniProt Accession #: Q96LI5; Q9UF92
• Molecular Weight: 63,001 Da
• NCBI Official Full Name: CCR4-NOT transcription complex subunit 6-like
• NCBI Official Synonym Full Names: CCR4-NOT transcription complex, subunit 6-like
• NCBI Official Symbol: CNOT6L
• NCBI Official Synonym Symbols: CCR4b
• NCBI Protein Information: CCR4-NOT transcription complex subunit 6-like; carbon catabolite repressor protein 4 homolog B
• UniProt Protein Name: CCR4-NOT transcription complex subunit 6-like
• UniProt Gene Name: CNOT6L
• UniProt Synonym Gene Names: CCR4B
• UniProt Entry Name: CNO6L_HUMAN

Uniprot Description

CNOT6L: Plays a role in the deadenylation of mRNAs in the cytoplasm. Has 3'-5' poly(A) exoribonuclease activity for synthetic poly(A) RNA substrate. May be involved in the deadenylation-dependent degradation of mRNAs through the 3'-UTR AU-rich element-mediated mechanism. Involved in deadenylation- dependent degradation of CDKN1B mRNA. Belongs to the CCR4/nocturin family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Hydrolase; EC 3.1.13.4

Chromosomal Location of Human Ortholog: 4q13.3

Cellular Component: cytoplasm; cytosol; nucleus; CCR4-NOT complex

Molecular Function: protein binding; poly(A)-specific ribonuclease activity; metal ion binding

Biological Process: regulation of translation; poly(A) tail shortening; transcription, DNA-dependent; regulation of transcription, DNA-dependent; positive regulation of cell proliferation; RNA-mediated gene silencing; gene expression; mRNA catabolic process, deadenylation-dependent decay