Ferric/cupric reductase transmembrane component 1 (FRE1) Recombinant Protein | FRE1 recombinant protein

Recombinant Saccharomyces cerevisiae Ferric/cupric reductase transmembrane component 1 (FRE1)

• Purity: Greater or equal to 85% purity as determined by SDS-PAGE.
• Synonyms: Ferric/cupric reductase transmembrane component 1 (FRE1); Recombinant Saccharomyces cerevisiae Ferric/cupric reductase transmembrane component 1 (FRE1); FRE1 recombinant protein

• Host: E Coli or Yeast or Baculovirus or Mammalian Cell
• Purity/Purification: Greater or equal to 85% purity as determined by SDS-PAGE.
• Form/Format: Lyophilized or liquid (Format to be determined during the manufacturing process)
• Sequence Positions: 23-686
• Sequence: TLISTSCISQAALYQFGCSSKSKSCYCKNINWLGSVTACAYENSKSNKTLDSALMKLASQCSSIKVYTLEDMKNIYLNASNYLRAPEKSDKKTVVSQPLMANETAYHYYYEENYGIHLNLMRSQWCAWGLVFFWVAVLTAATILNILKRVFGKNIMANSVKKSLIYPSVYKDYNERTFYLWKRLPFNFTTRGKGLVVLIFVILTILSLSFGHNIKLPHPYDRPRWRRSMAFVSRRADLMAIALFPVVYLFGIRNNPFIPITGLSFSTFNFYHKWSAYVCFMLAVVHSIVMTASGVKRGVFQSLVRKFYFRWGIVATILMSIIIFQSEKVFRNRGYEIFLLIHKAMNIMFIIAMYYHCHTLGWMGWIWSMAGILCFDRFCRIVRIIMNGGLKTATLSTTDDSNVIKISVKKPKFFKYQVGAFAYMYFLSPKSAWFYSFQSHPFTVLSERHRDPNNPDQLTMYVKANKGITRVLLSKVLSAPNHTVDCKIFLEGPYGVTVPHIAKLKRNLVGVAAGLGVAAIYPHFVECLRLPSTDQLQHKFYWIVNDLSHLKWFENELQWLKEKSCEVSVIYTGSSVEDTNSDESTKGFDDKEESEITVECLNKRPDLKELVRSEIKLSELENNNITFYSCGPATFNDDFRNAVVQGIDSSLKIDVELEEESFTW
• Sequence Length: 686

• Species: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
• Preparation and Storage: Store at -20 degree C. For extended storage, store at -20 or -80 degree C.

Related Product Information for FRE1 recombinant protein

FRE1

NCBI and Uniprot Product Information

• NCBI GI #: 6323243
• NCBI GeneID: 850911
• NCBI Accession #: NP_013315.1
• NCBI GenBank Nucleotide #: NM_001182101.1
• UniProt Accession #: P32791; D6VYL5
• Molecular Weight: 78,854 Da
• NCBI Official Full Name: Fre1p
• NCBI Official Symbol: FRE1
• NCBI Protein Information: Fre1p
• UniProt Protein Name: Ferric/cupric reductase transmembrane component 1
• Protein Family: Ferric/cupric reductase transmembrane component
• UniProt Gene Name: FRE1
• UniProt Entry Name: FRE1_YEAST

Uniprot Description

Function: Metalloreductase responsible for reducing extracellular iron and copper prior to import. Catalyzes the reductive uptake of Fe3+-salts and Fe3+ bound to catecholate or hydroxamate siderophores. Fe3+ is reduced to Fe2+, which then dissociates from the siderophore and can be imported by the high-affinity Fe2+ transport complex in the plasma membrane. Also participates in Cu2+ reduction and Cu+ uptake. Ref.1 Ref.6 Ref.7 Ref.8 Ref.10 Ref.15 Ref.16

Catalytic activity: 2 Fe2+ + NADP+ = 2 Fe3+ + NADPH.

Cofactor: FAD

Probable.Heme.

Enzyme regulation: Inhibited by nitric oxide (NO). Ref.18

Subcellular location: Cell membrane; Multi-pass membrane protein Ref.7 Ref.21.

Induction: By transription factor MAC1 upon copper deprivation and by transcription factor AFT1 upon iron deprivation. Ref.1 Ref.5 Ref.6 Ref.10 Ref.11 Ref.12 Ref.14 Ref.18 Ref.20

Biotechnological use: Responsible for the reduction of the azo bond of azo dyes, making yeasts efficient azo dye decolorizers. Ref.19

Miscellaneous: Present with 892 molecules/cell in log phase SD medium.

Sequence similarities: Belongs to the ferric reductase (FRE) family.Contains 1 FAD-binding FR-type domain.Contains 1 ferric oxidoreductase domain.