Folh1 recombinant protein

Folh1, 45-752aa, Mouse, His tag, Baculovirus

Cat. Num. AAA206363

• Gene Names: Folh1; GCP2; mopsm
• Applications: SDS-Page
• Purity: > 85% by SDS-PAGE
• Synonyms: Folh1; 45-752aa; Mouse; His tag; Baculovirus; Glutamate carboxypeptidase 2 isoform 1; GCP2; mopsm; Folh1 recombinant protein

• Host: Baculovirus
• Purity/Purification: > 85% by SDS-PAGE
• Form/Format: Liquid. In phosphate buffered saline (pH 7.4) containing 10% glycerol.
• Concentration: 0.5 mg/ml (determined by Absorbance at 280nm) (varies by lot)
• Sequence: ADPKPSNEAT GNVSHSGMKK EFLHELKAEN IKKFLYNFTR TPHLAGTQNN FELAKQIHDQ WKEFGLDLVE LSHYDVLLSY PNKTHPNYIS IINEDGNEIF KTSLSEQPPP GYENISDVVP PYSAFSPQGT PEGDLVYVNY ARTEDFFKLE REMKISCSGK IVIARYGKVF RGNMVKNAQL AGAKGMILYS DPADYFVPAV KSYPDGWNLP GGGVQRGNVL NLNGAGDPLT PGYPANEHAY RHELTNAVGL PSIPVHPIGY DDAQKLLEHM GGPAPPDSSW KGGLKVPYNV GPGFAGNFST QKVKMHIHSY TKVTRIYNVI GTLKGALEPD RYVILGGHRD AWVFGGIDPQ SGAAVVHEIV RSFGTLKKKG RRPRRTILFA SWDAEEFGLL GSTEWAEEHS RLLQERGVAY INADSSIEGN YTLRVDCTPL MYSLVYNLTK ELQSPDEGFE GKSLYDSWKE KSPSPEFIGM PRISKLGSGN DFEVFFQRLG IASGRARYTK NWKTNKVSSY PLYHSVYETY ELVVKFYDPT FKYHLTVAQV RGAMVFELAN SIVLPFDCQS YAVALKKYAD TIYNISMKHP QEMKAYMISF DSLFSAVNNF TDVASKFNQR LQELDKSNPI LLRIMNDQLM YLERAFIDPL GLPGRPFYRH IIYAPSSHNK YAGESFPGIY DALFDISSKV NASKAWNEVK RQISIATFTV QAAAETLREV AHHHHHH
• Sequence Length: 752

• Applicable Applications for Folh1 recombinant protein: SDS-PAGE
• Antigen Species: Mouse
• Tag Information: His-tag
• Endotoxin Level: < 1.0 EU per 1 microgram of protein (determined by LAL method)
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for Folh1 recombinant protein

Folh1, as known as glutamate carboxypeptidase 2, is a single pass type 2 membrane protein which belongs to the peptidase M28 family and M28B subfamily. This protein is most highly expressed in prostate epithelium. It is detected in urinary bladder, kidney, testis, ovary, live, stomach, small intestine colon, and the capillary endothelium of a variety of tumors. Thus, it shows a promising role in directed imaging and therapy of recurrent of metastatic disease. Recombinant mouse Folh1, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Categories/Family for Folh1 recombinant protein

Signal Transduction; Metabolism

References

Rahn KA., et al, (2012) Proc. Natl. Acad. Sci. U.S.A. 109:20101-20106.; Schaevitz LR., et al, (2012) Dev Neurobiol 72:891-905.

NCBI and Uniprot Product Information

• NCBI GI #: 228008367
• NCBI GeneID: 53320
• NCBI Accession #: NP_058050
• NCBI GenBank Nucleotide #: NM_016770.3
• UniProt Accession #: O35409; Q0VDM5; Q9DCC2
• Molecular Weight: 80.5kDa (717aa) 70-100kDa (SDS-PAGE under reducing conditions)
• NCBI Official Full Name: glutamate carboxypeptidase 2 isoform 1
• NCBI Official Synonym Full Names: folate hydrolase 1
• NCBI Official Symbol: Folh1
• NCBI Official Synonym Symbols: GCP2; mopsm
• NCBI Protein Information: glutamate carboxypeptidase 2
• UniProt Protein Name: Glutamate carboxypeptidase 2
• Protein Family: Glutamate carboxypeptidase
• UniProt Gene Name: Folh1
• UniProt Synonym Gene Names: Mopsm; Naalad1; FGCP; GCPII; mGCP; NAALADase I

Uniprot Description

Has both folate hydrolase and N-acetylated-alpha-linked-acidic dipeptidase (NAALADase) activity. Has a preference for tri-alpha-glutamate peptides (). In the intestine, required for the uptake of folate. In the brain, modulates excitatory neurotransmission through the hydrolysis of the neuropeptide, N-aceylaspartylglutamate (NAAG), thereby releasing glutamate.