Mouse Fibronectin ELISA Kit | FN1 elisa kit

Mouse Fibronectin PicoKine Fast ELISA Kit

Cat. Num. AAA1752713

• Gene Names: Fn1; Fn; Fn-1; E330027I09
• Reactivity: Mouse
• Synonyms: Fibronectin; Mouse Fibronectin PicoKine Fast ELISA Kit; Mouse Fibronectin; FN; Anastellin; Fn1; FN1 elisa kit

• Reactivity: Mouse
• Specificity: Natural and recombinant Mouse Fn1
• Sequence Length: 2477

• Samples: Cell culture supernates, serum and plasma(heparin, EDTA, citrate)
• Detection Range: 156pg/ml-10000pg/ml
• Sensitivity: <15pg/ml
• Intra-assay Precision: Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested on one plate to assess intra-assay precision.
• Inter-assay Precision: Inter-Assay Precision (Precision accross assays):Three samples of known concentration were tested in separate assays to assess inter-assay precision.
• Preparation and Storage: Store at 4 degree C for 6 months. Store at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for FN1 elisa kit

Principle of the Assay: The Picokine™ Mouse Fn1 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Mouse Fn1 with a 96-well strip plate that is pre-coated with antibody specific for Fn1. The detection antibody is a biotinylated antibody specific for Fn1. The capture antibody is monoclonal antibody from rat, the detection antibody is polyclonal antibody from goat. The kit contains recombinant Mouse Fn1 with immunogen: Expression system for standard: from plasma. The kit is analytically validated with ready to use reagents. To measure Mouse Fn1, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The density of the yellow product is linearly propotional to Mouse Fn1 in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Mouse Fn1 in the sample.!!Background/Introduction: Fibronectin(FN) also known as LETS, is identified on the surfFN of fibroblasts by labeling with radioactive compounds or specific antibodies. Fibronectin is a 430,000-dalton dimeric glycoprotein that exists in 2 forms, termed cellular and plasma fibronectin. Cellular and plasma fibronectins are heterodimers consisting of similar but not identical polypeptides. These two forms of FN differ in biologic activity. Fibronectins bind cell surfFNs and various compounds including collagen, fibrin, heparin, DNA, and actin. Because fibronectin stimulates endocytosis in several systems and promotes the clearance of particulate material from the circulation, it could function in the clearance of C1q-coated material such as immune complexes or cellular debris. Fibronectins are involved in cell adhesion, cell motility, opsonization, would healing, and maintenance of cell shape. LETS, encoded on chromosome 8, is responsible for the LETS protein expression in humans. Because LETS has been implicated in tumorigenicity and cellular transformation, it is of interest that rearrangement or modifications in the number of chromosome 8 have been associated with certain forms of cancer.

NCBI and Uniprot Product Information

• NCBI GI #: 46849812
• NCBI GeneID: 14268
• NCBI Accession #: NP_034363.1
• NCBI GenBank Nucleotide #: NP_034363.1
• UniProt Accession #: P11276
• NCBI Official Full Name: fibronectin isoform a
• NCBI Official Synonym Full Names: fibronectin 1
• NCBI Official Symbol: Fn1
• NCBI Official Synonym Symbols: Fn; Fn-1; E330027I09
• NCBI Protein Information: fibronectin
• UniProt Protein Name: Fibronectin
• Protein Family: Fibronectin
• UniProt Gene Name: Fn1
• UniProt Synonym Gene Names: FN
• UniProt Entry Name: FINC_MOUSE

Uniprot Description

FN1: Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin. Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape. Defects in FN1 are the cause of glomerulopathy with fibronectin deposits type 2 (GFND2); also known as familial glomerular nephritis with fibronectin deposits or fibronectin glomerulopathy. GFND is a genetically heterogeneous autosomal dominant disorder characterized clinically by proteinuria, microscopic hematuria, and hypertension that leads to end-stage renal failure in the second to fifth decade of life. 15 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Secreted; Cell adhesion; Motility/polarity/chemotaxis

Cellular Component: extracellular matrix; extracellular space; proteinaceous extracellular matrix; fibrinogen complex; apical plasma membrane; ER-Golgi intermediate compartment; extracellular region; basement membrane; basal lamina

Molecular Function: integrin binding; heparin binding; protein binding; protease activator activity; protease binding; mercury ion binding

Biological Process: integrin activation; extracellular matrix organization and biogenesis; wound healing; positive regulation of chemotaxis; cell-matrix adhesion; positive regulation of axon extension; glial cell migration; cell activation; regulation of cell shape; cell-substrate junction assembly; acute-phase response; calcium-independent cell-matrix adhesion; peptide cross-linking; angiogenesis; cell adhesion; positive regulation of cell migration; negative regulation of apoptosis