Mouse VEGFR3/FLT4 ELISA Kit | FLT4 elisa kit

Mouse VEGFR3/FLT4 PicoKine ELISA Kit

Cat. Num. AAA177147

• Gene Names: Flt4; Chy; Flt-4; VEGFR3; VEGFR-3; AI323512
• Reactivity: Mouse
• Synonyms: VEGFR3/FLT4; Mouse VEGFR3/FLT4 PicoKine ELISA Kit; Vascular endothelial growth factor receptor 3; fms-related tyrosine kinase 4; EC 2.7.10.1; flt 4; FLT-4; FLT4; FLT41; Fms related tyrosine kinase 4; Fms-like tyrosine kinase 4; LMPH1A; PCL; Soluble VEGFR3 variant 1; Soluble VEGFR3 variant 2; Soluble VEGFR3 variant 3; Tyrosine protein kinase receptor FLT4; Tyrosine-protein kinase receptor FLT4; vascular endothelial growth factor receptor 3; Vascular endothelial growth factor receptor 3 precursor; VEGF R3; VEGFR 3; VEGFR-3; VEGFR3; VGFR3_HUMAN; FLT4 elisa kit

• Reactivity: Mouse
• Sequence Length: 1363

• Samples: Cell culture supernates and serum
• Assay Type: Sandwich
• Detection Range: 156pg/ml-10,000pg/ml
• Sensitivity: <10pg/ml
• Immunogen: NSO, Y25?D770
• Reactivity Note: Mouse. There is no detectable cross-reactivity with other relevant proteins.
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Typical Testing Data/Standard Curve (for reference only)

(Mouse VEGFR3/FLT4 PicoKine ELISA Kit standard curve )

Related Product Information for FLT4 elisa kit

Description: Sandwich ELISA kit of Quantitative Detection for Mouse VEGFR3/FLT4
Background: Fms-related tyrosine kinase 4, also known as FLT4 or VEGFR3, is a protein which in humans is encoded by the FLT4 gene. It is mapped to 5q35.3. This gene encodes a tyrosine kinase receptor for vascular endothelial growth factors C and D. The protein is thought to be involved in lymphangiogenesis and maintenance of the lymphatic endothelium. FLT4 has an essential role in the development of the embryonic cardiovascular system before the emergence of the lymphatic vessels. It has been found that FLT4, which provides proangiogenic signaling when expressed on endothelium, may also have antiangiogenic properties when expressed at an avascular site by nonendothelial cells. FLT4 is also regarded as a regulator of vascular network formation.

NCBI and Uniprot Product Information

• NCBI GI #: 544322
• NCBI GeneID: 14257
• UniProt Accession #: P35917
• Molecular Weight: 153,016 Da
• NCBI Official Full Name: Vascular endothelial growth factor receptor 3
• NCBI Official Synonym Full Names: FMS-like tyrosine kinase 4
• NCBI Official Symbol: Flt4
• NCBI Official Synonym Symbols: Chy; Flt-4; VEGFR3; VEGFR-3; AI323512
• NCBI Protein Information: vascular endothelial growth factor receptor 3; chylous ascites; receptor protein tyrosine kinase; tyrosine-protein kinase receptor FLT4; vascular endothelial growth factor receptor-3
• UniProt Protein Name: Vascular endothelial growth factor receptor 3
• Protein Family: Vascular endothelial growth factor receptor
• UniProt Gene Name: Flt4
• UniProt Synonym Gene Names: Flt-4; Vegfr3; VEGFR-3; FLT-4
• UniProt Entry Name: VGFR3_MOUSE

Uniprot Description

VEGFR3: Tyrosine-protein kinase that acts as a cell-surface receptor for VEGFC and VEGFD, and plays an essential role in adult lymphangiogenesis and in the development of the vascular network and the cardiovascular system during embryonic development. Promotes proliferation, survival and migration of endothelial cells, and regulates angiogenic sprouting. Signaling by activated FLT4 leads to enhanced production of VEGFC, and to a lesser degree VEGFA, thereby creating a positive feedback loop that enhances FLT4 signaling. Modulates KDR signaling by forming heterodimers. The secreted isoform 3 may function as a decoy receptor for VEGFC and/or VEGFD and play an important role as a negative regulator of VEGFC-mediated lymphangiogenesis and angiogenesis. Binding of vascular growth factors to isoform 1 or isoform 2 leads to the activation of several signaling cascades; isoform 2 seems to be less efficient in signal transduction, because it has a truncated C-terminus and therefore lacks several phosphorylation sites. Mediates activation of the MAPK1/ERK2, MAPK3/ERK1 signaling pathway, of MAPK8 and the JUN signaling pathway, and of the AKT1 signaling pathway. Phosphorylates SHC1. Mediates phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3- kinase. Promotes phosphorylation of MAPK8 at 'Thr-183' and 'Tyr- 185', and of AKT1 at 'Ser-473'. Interacts with VEGFC and VEGFD. Monomer in the absence of bound VEGFC or VEGFD. Homodimer in the presence of bound VEGFC or VEGFD. Can also form a heterodimer with KDR. Interacts with PTPN14; the interaction is enhanced by stimulation with VEGFC. Interacts with CRK, GRB2, PTK2/FAK1, SHC1, PIK3R1 and PTPN11/SHP- 2. Identified in a complex with SRC and ITGB1. Detected in endothelial cells. Widely expressed. Detected in fetal spleen, lung and brain. Detected in adult liver, muscle, thymus, placenta, lung, testis, ovary, prostate, heart, and kidney. Present in an inactive conformation in the absence of bound ligand. Binding of VEGFC or VEGFD leads to dimerization and activation by autophosphorylation on tyrosine residues. Inhibited by MAZ51. Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; EC 2.7.10.1; Protein kinase, tyrosine (receptor); Kinase, protein; Protein kinase, TK; TK group; VEGFR family

Cellular Component: membrane; integral to plasma membrane; cytoplasm; integral to membrane; plasma membrane; nucleus; receptor complex

Molecular Function: transferase activity; vascular endothelial growth factor receptor activity; protein binding; protein-tyrosine kinase activity; growth factor binding; transferase activity, transferring phosphorus-containing groups; nucleotide binding; kinase activity; transmembrane receptor protein tyrosine kinase activity; protein phosphatase binding; ATP binding; protein kinase activity

Biological Process: peptidyl-tyrosine phosphorylation; protein amino acid autophosphorylation; positive regulation of JNK cascade; positive regulation of cell growth; protein amino acid phosphorylation; vasculature development; positive regulation of MAPKKK cascade; lymphangiogenesis; positive regulation of cell proliferation; blood vessel morphogenesis; positive regulation of endothelial cell proliferation; angiogenesis; sprouting angiogenesis; positive regulation of protein amino acid phosphorylation; vascular endothelial growth factor receptor signaling pathway; lymph vessel development; phosphorylation; transmembrane receptor protein tyrosine kinase signaling pathway; negative regulation of apoptosis