VEGFR1 / FLT-1 recombinant protein

Recombinant Rat VEGFR1 / FLT-1 Protein (His tag)

Cat. Num. AAA2547465

• Gene Names: Flt1; FLT-1; VEGFR-1
• Purity: > 90 % as determined by SDS-PAGE
• Synonyms: VEGFR1 / FLT-1; Recombinant Rat VEGFR1 / FLT-1 Protein (His tag); FLT1; Flt-1; Vegfr1; VEGFR1 / FLT-1 recombinant protein

• Host: Human Cells
• Purity/Purification: > 90 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH 7.4
• Sequence Length: 1336

• Application Notes: The recombinant rat FLT1 comprises 747 amino acids and predicts a molecular mass of 84 kDa. The apparent molecular mass of the recombinant protein is approximately 108-114 kDa in SDS-PAGE under reducing conditions due to glycosylation.
• Predicted N Terminal: Tyr 23
• Endotoxin: < 1.0 EU per mug of the protein as determined by the LAL method
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for VEGFR1 / FLT-1 recombinant protein

Background: Vascular endothelial growth factor receptor 1, also known as VEGFR-1, Fms-like tyrosine kinase 1, Tyrosine-protein kinase FRT, Tyrosine-protein kinase receptor FLT, Vascular permeability factor receptor and FLT1, is a single-pass type I membrane protein and secreted protein which belongs to the protein kinase superfamily, Tyr protein kinase family and CSF-1/PDGF receptor subfamily. VEGFR-1 / FLT1 contains seven Ig-like C2-type (immunoglobulin-like) domains and one protein kinase domain. VEGFR-1 / FLT1 is expressed mostly in normal lung, but also in placenta, liver, kidney, heart and brain tissues. It is specifically expressed in most of the vascular endothelial cells, and also expressed in peripheral blood monocytes. VEGFR-1 / FLT1 is not expressed in tumor cell lines. VEGFR-1 / FLT1 is an essential receptor tyrosine kinase that regulates mammalian vascular development and embryogenesis. EGF-induced angiogenesis requires inverse regulation of VEGFR-1 and VEGFR-2 in tumor-associated endothelial cells. VEGFR-1 / FLT1 is a receptor for VEGF, VEGFB and PGF. It has a tyrosine-protein kinase activity. The VEGF-kinase ligand/receptor signaling system plays a key role in vascular development and regulation of vascular permeability.

Description: A DNA sequence encoding the rat FLT1 (P53767) (Met1-Glu758) was expressed with a polyhistidine tag at the C-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 1718161
• NCBI GeneID: 54251
• UniProt Accession #: P53767
• Molecular Weight: 150,250 Da
• NCBI Official Full Name: Vascular endothelial growth factor receptor 1
• NCBI Official Synonym Full Names: FMS-related tyrosine kinase 1
• NCBI Official Symbol: Flt1
• NCBI Official Synonym Symbols: FLT-1; VEGFR-1
• NCBI Protein Information: vascular endothelial growth factor receptor 1
• UniProt Protein Name: Vascular endothelial growth factor receptor 1
• UniProt Gene Name: Flt1
• UniProt Synonym Gene Names: Flt-1; Vegfr1; VEGFR-1; FLT-1

NCBI Description

tyrosine kinase receptor for vascular endothelial growth factor; may play a role in cell proliferation and cell survival [RGD, Feb 2006]

Uniprot Description

VEGFR1: a receptor tyrosine kinase of the VEGFR family. Receptor for VEGF, VEGFB and PGF. The VEGF-kinase ligand/receptor signaling system plays a key role in vascular development and regulation of vascular permeability. Two splice variant isoforms have been described. Isoform SFlt1 may have an inhibitory role in angiogenesis.

Protein type: EC 2.7.10.1; Kinase, protein; Membrane protein, integral; Protein kinase, TK; Protein kinase, tyrosine (receptor); TK group; VEGFR family

Chromosomal Location of Human Ortholog: 12p11

Cellular Component: extracellular space; focal adhesion; integral to plasma membrane; nucleus; plasma membrane; receptor complex

Molecular Function: ATP binding; growth factor binding; identical protein binding; receptor signaling protein tyrosine kinase activity; transmembrane receptor protein tyrosine kinase activity; vascular endothelial growth factor receptor activity

Biological Process: activation of MAPKK activity; aging; angiogenesis; blood vessel morphogenesis; cell migration; embryonic morphogenesis; female pregnancy; intracellular receptor-mediated signaling pathway; monocyte chemotaxis; patterning of blood vessels; peptidyl-tyrosine phosphorylation; positive regulation of angiogenesis; positive regulation of cell migration; positive regulation of cell proliferation; positive regulation of fibroblast proliferation; positive regulation of MAP kinase activity; positive regulation of MAPKKK cascade; positive regulation of phosphoinositide 3-kinase activity; positive regulation of phosphoinositide 3-kinase cascade; positive regulation of smooth muscle cell proliferation; positive regulation of vascular endothelial growth factor receptor signaling pathway; post-embryonic camera-type eye morphogenesis; protein amino acid autophosphorylation; protein amino acid phosphorylation; regulation of smooth muscle contraction; response to activity; response to estradiol stimulus; response to ethanol; response to hypoxia; sprouting angiogenesis; transmembrane receptor protein tyrosine kinase signaling pathway; vascular endothelial growth factor receptor signaling pathway