Vascular Endothelial Growth Factor Receptor 1 (VEGFR1) Recombinant Protein | VEGFR1 recombinant protein

Recombinant Vascular Endothelial Growth Factor Receptor 1 (VEGFR1)

Cat. Num. AAA2010855

• Gene Names: Flt1; Flt-1; sFlt1; VEGFR1; VEGFR-1; AI323757
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Vascular Endothelial Growth Factor Receptor 1 (VEGFR1); Recombinant Vascular Endothelial Growth Factor Receptor 1 (VEGFR1); VEGFR1 recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with N-terminal His-Tag, its sequence is listed below.; MGHHHHHHSGSEF-SKLKVPELSL KGTQHVMQAG QTLFLKCRGE AAHSWSLPTT VSQEDKRLSI TPPSACGRDN RQFCSTLTLD TAQANHTGLY TCRYLPTSTS KKKKAESSIY IFVSDAGSPF IEMHTDIPKL VHMTEGRQLI IPCRVTSPNV TVTLKKFPFD TLTPDGQRIT WDSRRGFIIA NATYKEIGLL NCEATVNGHL YQTNYLTHRQ TNTILDVQIR PPSPVRLLHG QTLVLNCTAT TELNTRVQMS WNYPGKATKR ASIRQRIDRS HSHNNVFHSV LKINNVESRD KGLYTCRVKS GSSFQSFNTS VHV
• Sequence Length: 1333

• Applicable Applications for VEGFR1 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Mus musculus (Mouse)
• Expression System: Prokaryotic expression
• Residues: Ser27~Val329 (Accession # P35969) with N-terminal His-Tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

Sequence Information

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 34328180
• NCBI GeneID: 14254
• NCBI Accession #: NP_034358.2
• NCBI GenBank Nucleotide #: NM_010228.3
• UniProt Accession #: P35969; O55094; Q61517
• Molecular Weight: 35.5kDa
• NCBI Official Full Name: vascular endothelial growth factor receptor 1
• NCBI Official Synonym Full Names: FMS-like tyrosine kinase 1
• NCBI Official Symbol: Flt1
• NCBI Official Synonym Symbols: Flt-1; sFlt1; VEGFR1; VEGFR-1; AI323757
• NCBI Protein Information: vascular endothelial growth factor receptor 1; embryonic receptor kinase 2; tyrosine-protein kinase receptor FLT; vascular permeability factor receptor; vascular endothelial growth factor receptor-1
• UniProt Protein Name: Vascular endothelial growth factor receptor 1
• UniProt Gene Name: Flt1
• UniProt Synonym Gene Names: Emrk2; Flt; Vegfr1; VEGFR-1; FLT-1
• UniProt Entry Name: VGFR1_MOUSE

Uniprot Description

VEGFR1: a receptor tyrosine kinase of the VEGFR family. Receptor for VEGF, VEGFB and PGF. The VEGF-kinase ligand/receptor signaling system plays a key role in vascular development and regulation of vascular permeability. Two splice variant isoforms have been described. Isoform SFlt1 may have an inhibitory role in angiogenesis.

Protein type: EC 2.7.10.1; Kinase, protein; Membrane protein, integral; Protein kinase, TK; Protein kinase, tyrosine (receptor); TK group; VEGFR family

Cellular Component: focal adhesion; membrane; integral to plasma membrane; integral to membrane; plasma membrane; endosome; receptor complex

Molecular Function: transferase activity; vascular endothelial growth factor receptor activity; identical protein binding; protein-tyrosine kinase activity; growth factor binding; transferase activity, transferring phosphorus-containing groups; nucleotide binding; transmembrane receptor protein tyrosine kinase activity; kinase activity; ATP binding; protein kinase activity

Biological Process: cell migration; peptidyl-tyrosine phosphorylation; multicellular organismal development; protein amino acid autophosphorylation; positive regulation of phosphoinositide 3-kinase activity; chemotaxis; protein amino acid phosphorylation; positive regulation of vascular endothelial growth factor receptor signaling pathway; positive regulation of phosphoinositide 3-kinase cascade; patterning of blood vessels; monocyte chemotaxis; positive regulation of MAP kinase activity; positive regulation of angiogenesis; positive regulation of MAPKKK cascade; response to hypoxia; blood vessel morphogenesis; embryonic morphogenesis; sprouting angiogenesis; angiogenesis; cell differentiation; vascular endothelial growth factor receptor signaling pathway; phosphorylation; transmembrane receptor protein tyrosine kinase signaling pathway; positive regulation of cell migration