FLRT2 recombinant protein

Recombinant Human FLRT2 Protein (His tag)

Cat. Num. AAA2545824

• Purity: > 98 % as determined by SDS-PAGE
• Synonyms: FLRT2; Recombinant Human FLRT2 Protein (His tag); UNQ232/PRO265; KIAA0405; FLRT2 recombinant protein

• Host: Human Cells
• Purity/Purification: > 98 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH 7.4
• Sequence Length: 660

• Application Notes: The secreted recombinant human FLRT2 comprises 515 amino acids and has a calculated molecular mass of 57.7 kDa. The apparent molecular mass of the recombinant protein is approximately 75 kDa in SDS-PAGE under reducing conditions due to glycosylation.
• Predicted N Terminal: Cys 36
• Endotoxin: < 1.0 EU per mug of the protein as determined by the LAL method
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for FLRT2 recombinant protein

Background: Fibronectin Leucine-Rich Transmembrane (FLRT) proteins are glycosylated membrane proteins expressed at the cell surface which localise in a homophilic manner to cell-cell contacts expressing the focal adhesion marker vinculin. FLRT1, FLRT2, and FLRT3, the three genes encode putative type I transmembrane proteins, each containing 10 leucine-rich repeats (LRR), a type III fibronectin (FN) domain, followed by the transmembrane region, and a short cytoplasmic tail. FLRT family members may function in cell adhesion and/or receptor signalling. Each member of the FLRT family has a distinct, highly regulated expression pattern, as was seen for the NLRR family. FLRT2 is expressed in a subset of the sclerotome, adjacent to the region that forms the syndetome, suggesting that interaction with FGF signalling may be a general property of FLRT proteins. All FLRTs can interact with FGFR1 and FLRTs can be induced by the activation of FGF signalling by FGF-2. FLRT proteins have a dual role, promoting FGF signalling and modulating homotypic cell adhesion. FLRT2 played critical roles in craniofacial development, and it was also present in the vomero-nasal organ, mandibular primodia, and the posterior aspects of the unfused and fused secondary palatal shelves.

Description: A DNA sequence encoding the human FLRT2 (O43155) extracellular domain (Met 1-Ser 539) was expressed, with a C-terminal polyhistidine tag.

NCBI and Uniprot Product Information

• NCBI GI #: 1071034772
• NCBI GeneID: 23768
• NCBI Accession #: NP_001333072.1
• NCBI GenBank Nucleotide #: NM_001346143.1
• UniProt Accession #: O43155; A0AV84; B7ZLP3
• Molecular Weight: 74,049 Da
• NCBI Official Full Name: leucine-rich repeat transmembrane protein FLRT2
• NCBI Official Synonym Full Names: fibronectin leucine rich transmembrane protein 2
• NCBI Official Symbol: FLRT2
• NCBI Protein Information: leucine-rich repeat transmembrane protein FLRT2
• UniProt Protein Name: Leucine-rich repeat transmembrane protein FLRT2
• Protein Family: Leucine-rich repeat transmembrane protein
• UniProt Gene Name: FLRT2
• UniProt Synonym Gene Names: KIAA0405

NCBI Description

This gene encodes a member of the fibronectin leucine rich transmembrane (FLRT) family of cell adhesion molecules, which regulate early embryonic vascular and neural development. The encoded type I transmembrane protein has an extracellular region consisting of an N-terminal leucine-rich repeat domain and a type 3 fibronectin domain, followed by a transmembrane domain and a short C-terminal cytoplasmic tail domain. It functions as both a homophilic cell adhesion molecule and a heterophilic chemorepellent through its interaction with members of the uncoordinated-5 receptor family. Proteolytic removal of the extracellular region controls the migration of neurons in the developing cortex. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2016]

Uniprot Description

FLRT2: May have a function in cell adhesion and/or receptor signaling.

Protein type: Extracellular matrix; Membrane protein, integral; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 14q31.3

Cellular Component: cytoplasm; focal adhesion; integral to plasma membrane; intercellular junction; plasma membrane

Molecular Function: protein kinase inhibitor activity

Biological Process: cytokine and chemokine mediated signaling pathway; fibroblast growth factor receptor signaling pathway; heart morphogenesis; negative regulation of JAK-STAT cascade; negative regulation of protein kinase activity