FLRT1 active protein

FLRT1 Protein, Human, Recombinant (His Tag)

Cat. Num. AAA8120806

• Gene Names: FLRT1; SPG68
• Purity: >96% as determined by SDS-PAGE
• Synonyms: FLRT1; FLRT1 Protein; Human; Recombinant (His Tag); Human FLRT1 Protein (His Tag); SPG68 Protein; fibronectin leucine rich transmembrane protein 1; FLRT1 active protein

• Host: HEK293 Cells
• Purity/Purification: >96% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Pro524

• Species: Human
• Activity: Measured by the ability of the immobilized protein to support the adhesion of Neuro?2A mouse neuroblastoma cells. When cells are added to coated plates(5ug/mL, 100uL/well), approximately 50%-70% will adhere after 1 hour at 37 degree C.
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Ile 21
• Tag: C-His
• Protein Construction: A DNA sequence encoding the human FLRT1 extracellular domain (Q9NZU1-1)(Met 1-Pro 524) was expressed, fused with a polyhistidine tag at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for FLRT1 active protein

Background: The three fibronectin leucine-rich repeat transmembrane (FLRT) proteins contain 10 leucine-rich repeats (LRR), a type III fibronectin (FN) domain, followed by the transmembrane region, and a short cytoplasmic tail. FLRT1 is expressed in kidney and brain, which is a target for tyrosine phosphorylation mediated by FGFR1 and implicates a non-receptor Src family kinase (SFK). All FLRTs can interact with FGFR1 and FLRTs can be induced by the activation of FGF signalling by FGF-2. The phosphorylation state of FLRT1, which is itself FGFR1 dependent, may play a critical role in the potentiation of FGFR1 signalling and may also depend on a SFK-dependent phosphorylation mechanism acting via the FGFR. This is consistent with an 'in vivo' role for FLRT1 regulation of FGF signalling via SFKs. Furthermore, the phosphorylation-dependent futile cycle mechanism controlling FGFR1 signalling is concurrently crucial for regulation of FLRT1-mediated neurite outgrowth. FLRT1, FLRT2 and FLRT3 are members of the fibronectin leucine rich transmembrane protein (FLRT) family. They may function in cell adhesion and/or receptor signalling. Their protein structures resemble small leucine-rich proteoglycans found in the extracellular matrix. FLRT3 shares 55% amino acid sequence identity with FLRT1.

References

Lacy SE, et al. (1999) Identification of FLRT1, FLRT2, and FLRT3: a novel family of transmembrane leucine-rich repeat proteins. Genomics. 62(3): 417-26.Haines BP, et al. (2006) Regulated expression of FLRT genes implies a functional role in the regulation of FGF signalling during mouse development. Dev Biol. 297(1): 14-25.Maretto S, et al. (2008) Ventral closure, headfold fusion and definitive endoderm migration defects in mouse embryos lacking the fibronectin leucine-rich transmembrane protein FLRT3. Dev Biol. 318(1): 184-93.Wheldon LM, et al. (2010) Critical role of FLRT1 phosphorylation in the interdependent regulation of FLRT1 function and FGF receptor signalling. PLoS One. 5(4): e10264.

NCBI and Uniprot Product Information

• NCBI GI #: 34577057
• NCBI GeneID: 23769
• NCBI Accession #: NP_037412
• NCBI GenBank Nucleotide #: NM_013280
• UniProt Accession #: Q9NZU1; Q8WVA2
• Molecular Weight: 71,358 Da
• NCBI Official Full Name: leucine-rich repeat transmembrane protein FLRT1
• NCBI Official Synonym Full Names: fibronectin leucine rich transmembrane protein 1
• NCBI Official Symbol: FLRT1
• NCBI Official Synonym Symbols: SPG68
• NCBI Protein Information: leucine-rich repeat transmembrane protein FLRT1; fibronectin-like domain-containing leucine-rich transmembrane protein 1
• UniProt Protein Name: Leucine-rich repeat transmembrane protein FLRT1
• Protein Family: Leucine-rich repeat transmembrane protein
• UniProt Gene Name: FLRT1
• UniProt Entry Name: FLRT1_HUMAN