Human Flotillin-1 ELISA Kit | FLOT1 elisa kit

Human Flotillin-1 ELISA Kit

Cat. Num. AAA9426742

• Reactivity: Human
• Synonyms: Flotillin-1; Human Flotillin-1 ELISA Kit; FLOT1; FLOT1 elisa kit

• Reactivity: Human
• Specificity: This assay recognizes recombinant and natural human Flotillin-1. No significant cross-reactivity or interference was observed.

• Samples: Serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids
• Detection Range: 0.15 ng/mL -10.0 ng/mL.
• Preparation and Storage: Store at 4 degree C

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for FLOT1 elisa kit

Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of human Flotillin-1 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to Flotillin-1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Flotillin-1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Flotillin-1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of Flotillin-1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 974141105
• NCBI GeneID: 10211
• NCBI Accession #: NP_001305804.1
• NCBI GenBank Nucleotide #: NM_001318875.1
• UniProt Accession #: O75955; Q969J8; Q9UHW1; Q9UNV8; B4DVY7
• Molecular Weight: 42,080 Da
• NCBI Official Full Name: flotillin-1 isoform 2
• NCBI Official Synonym Full Names: flotillin 1
• NCBI Official Symbol: FLOT1
• NCBI Protein Information: flotillin-1
• UniProt Protein Name: Flotillin-1
• Protein Family: Flotillin
• UniProt Gene Name: FLOT1

NCBI Description

This gene encodes an protein that localizes to the caveolae, which are small domains on the inner cell membranes. This protein plays a role in vesicle trafficking and cell morphology. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2016]

Uniprot Description

FLOT1: May act as a scaffolding protein within caveolar membranes, functionally participating in formation of caveolae or caveolae-like vesicles. Heterooligomeric complex of flotillin-1 and flotillin-2 and caveolin-1 and caveolin-2. Interacts with ECM29. Belongs to the band 7/mec-2 family. Flotillin subfamily.

Protein type: Adaptor/scaffold

Chromosomal Location of Human Ortholog: 6p21.33

Cellular Component: apical plasma membrane; basolateral plasma membrane; caveola; cell-cell adherens junction; cortical actin cytoskeleton; cytoplasmic vesicle; early endosome; endosome; flotillin complex; focal adhesion; integral to membrane; intercellular junction; lamellipodium; lipid raft; lysosomal membrane; membrane; microtubule organizing center; plasma membrane; signalosome; uropod

Molecular Function: protease binding; protein binding; protein heterodimerization activity

Biological Process: dsRNA transport; extracellular matrix disassembly; lipid raft formation; positive regulation of cytokine production; positive regulation of endocytosis; positive regulation of heterotypic cell-cell adhesion; positive regulation of interferon-beta production; positive regulation of protein amino acid phosphorylation; positive regulation of protein binding; positive regulation of skeletal muscle development; positive regulation of synaptic transmission, dopaminergic; positive regulation of toll-like receptor 3 signaling pathway; protein homooligomerization; protein stabilization; regulation of receptor internalization; regulation of toll-like receptor 4 signaling pathway