FKBP12 / FKBP1A recombinant protein

Recombinant Mouse FKBP12 / FKBP1A Protein (His tag)

Cat. Num. AAA2547070

• Gene Names: Fkbp1a; Fkbp; Fkbp1; FKBP12
• Purity: > 97 % as determined by SDS-PAGE
• Synonyms: FKBP12 / FKBP1A; Recombinant Mouse FKBP12 / FKBP1A Protein (His tag); RP23-419G21.5; FKBP12; FKBP12-T1; FKBP12-T2; Fkbp; Fkbp1; mFKBP1; mFKBP12; FKBP12 / FKBP1A recombinant protein

• Host: E Coli
• Purity/Purification: > 97 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH 7.4
• Sequence Length: 108

• Application Notes: The recombinant mouse FKBP1A consisting of 114 amino acids and has a calculated molecular mass of 12.7 kDa. rmFKBP1A migrates as an approximately 14 kDa band in SDS-PAGE under reducing conditions.
• Predicted N Terminal: Met
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for FKBP12 / FKBP1A recombinant protein

Background: FK506 binding protein 12 (FKBP12), also known as FKBP1, along with cyclophilin, are two major members of the immunophilin protein family who serve as receptors for the immunosuppressant drugs cyclosporin A and FK506. As a conserved molecules in many eukaryotes, FKBP12 has been characterized as a peptidyl-prolyl isomerase that catalyzes the transition between cis- and trans-proline residues, and is involved in several biochemical processes including protein folding, receptor signaling, protein trafficking and transcription. FKBP12 has attracted immense attention and its role in mediating the immunosuppressive functions. FKBP12 serves a dual role as a peptidyl-prolyl cis-trans isomerase and as a modulator of several cell signaling pathways. In one such a role, FKBP12 interacts with and regulates the functional state of the ryanodine Ca2+ channel receptor by altering protein conformation and coordinating multi-protein complex formation. Another physiological role of FKBP12 is an interactor and a regulator of the type I serine/threonine kinase receptors of TGF-beta superfamily. Current data, derived from detailed biochemical studies as well as from functional studies in various systems, suggest that FKBP12 functions as a "guardian" for the type I receptors to prevent them from leaky signaling under sub-optimal ligand concentrations, thereby providing a molecular "gradient reader" for TGF-beta family morphogens. This aspect of FKBP12 function may be critical for cellular responsiveness to morphogenetic gradients of the TGF-beta family members during early development, serving to assure the translation of different ligand concentrations into different signaling readouts. In addition, FKBP12 may be involved in neuronal or astrocytic cytoskeletal organization and in the abnormal metabolism of tau protein in Alzheimer's disease (AD) damaged neurons.

Description: A DNA sequence encoding the mouse FKBP1A (NP_032045.1) (Gly 2-Glu 108) was expressed, with a polyhistide tag at the N-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 6679803
• NCBI GeneID: 14225
• NCBI Accession #: NP_032045.1
• NCBI GenBank Nucleotide #: NM_008019.3
• UniProt Accession #: P26883; Q545E9
• Molecular Weight: 11,923 Da
• NCBI Official Full Name: peptidyl-prolyl cis-trans isomerase FKBP1A isoform 1
• NCBI Official Synonym Full Names: FK506 binding protein 1a
• NCBI Official Symbol: Fkbp1a
• NCBI Official Synonym Symbols: Fkbp; Fkbp1; FKBP12
• NCBI Protein Information: peptidyl-prolyl cis-trans isomerase FKBP1A
• UniProt Protein Name: Peptidyl-prolyl cis-trans isomerase FKBP1A
• UniProt Gene Name: Fkbp1a
• UniProt Synonym Gene Names: Fkbp1; PPIase FKBP1A; 12 kDa FKBP; FKBP-12; FKBP-1A

NCBI Description

This gene is a member of the immunophilin family. The encoded protein is a cis-trans prolyl isomerase that binds the immunosuppressants FK506 and rapamycin, and is associated with immunoregulation, protein folding, receptor signaling, protein trafficking and T-cell activation. It may modulate the calcium release activity of the ryanodine receptor Ryr1. It also interacts with the type I TGF-beta receptor. Disruption of this gene in mouse causes severe ventricular defects. Pseudogenes of this gene have been defined on chromosomes 4, 10, 14, and 16. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2014]

Uniprot Description

FKBP12: an immunophilin and peptidyl-prolyl cis-trans isomerase that binds to the immunosuppressant drugs FK506 (a.k.a. tacrolimus or Fujimycin) and rapamycin (a.k.a. Sirolimus). The FKBP12/rapamycin complex binds NFAT transcription factors and inhibits the induction of T cell genes including IL-2, IL-3, IL-4, TNF-alpha and GM-CSF. FK506 is used in treating patients after organ transplant and those suffering from autoimmune disorders. The immunosuppressant activity of FKBP12 is not apparently related to its prolyl isomerase activity. The FKBP12/rapamycin complex inhibits the mammalian target of rapamycin (mTOR) pathway by directly binding the mTOR Complex1 (mTORC1). mTORC1 contains Raptor, mLST8, and PRAS40, and interacts with DEPTOR, which inhibits its activity. mTOR, a S/T protein kinase, is a central regulator of cellular growth and metabolism. Its inhibition enhances the dependence on aerobic glycolysis in leukemic cells.

Protein type: EC 5.2.1.8; Isomerase

Chromosomal Location of Human Ortholog: 2|2 G3

Cellular Component: cytoplasm; cytosol; intracellular membrane-bound organelle; membrane; nerve terminal; sarcoplasmic reticulum membrane; Z disc

Molecular Function: activin binding; drug binding; enzyme binding; FK506 binding; Hsp70 protein binding; peptidyl-prolyl cis-trans isomerase activity; protein binding; protein homodimerization activity; signal transducer activity; SMAD binding; transforming growth factor beta receptor binding

Biological Process: cytokine and chemokine mediated signaling pathway; fibril organization and biogenesis; heart morphogenesis; muscle contraction; negative regulation of protein amino acid phosphorylation; negative regulation of protein phosphatase type 2B activity; positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of protein binding; positive regulation of protein ubiquitination; protein peptidyl-prolyl isomerization; regulation of activin receptor signaling pathway; regulation of immune response; regulation of protein localization; release of sequestered calcium ion into cytosol; response to caffeine; SMAD protein complex assembly; T cell proliferation; ventricular cardiac muscle morphogenesis