Rat Coagulation Factor IX ELISA Kit | FIX elisa kit

Rat Coagulation Factor IX ELISA Kit

Cat. Num. AAA764137

• Gene Names: F9; FIX; P19; PTC; HEMB; THPH8; F9 p22
• Reactivity: Rat
• Synonyms: Coagulation Factor IX; Rat Coagulation Factor IX ELISA Kit; FIX; FIX elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of FGF23. No significant cross-reactivity or interference between FGF23 and analogues was observed.
• Sequence Length: 461

• Samples: Serum, Plasma, Tissue Homogenates And Other Biological Fluids
• Assay Type: Sandwich
• Detection Range: 15.625-1000pg/ml
• Sensitivity: <9.375pg/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level FGF23 were tested 20 times on one plate, respectively. Intra-Assay: CV<8%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level FGF23 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<10%
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for FIX elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-FGF23 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-FGF23 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the FGF23 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of FGF23 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 4503649
• NCBI GeneID: 2158
• NCBI Accession #: NP_000124.1
• NCBI GenBank Nucleotide #: NM_000133.3
• Molecular Weight: 47,618 Da
• NCBI Official Full Name: coagulation factor IX isoform 1 preproprotein
• NCBI Official Synonym Full Names: coagulation factor IX
• NCBI Official Symbol: F9
• NCBI Official Synonym Symbols: FIX; P19; PTC; HEMB; THPH8; F9 p22
• NCBI Protein Information: coagulation factor IX
• UniProt Protein Name: Coagulation factor IX
• Protein Family: Coagulation factor
• UniProt Gene Name: F9
• UniProt Synonym Gene Names: PTC
• UniProt Entry Name: FA9_HUMAN

NCBI Description

This gene encodes vitamin K-dependent coagulation factor IX that circulates in the blood as an inactive zymogen. This factor is converted to an active form by factor XIa, which excises the activation peptide and thus generates a heavy chain and a light chain held together by one or more disulfide bonds. The role of this activated factor IX in the blood coagulation cascade is to activate factor X to its active form through interactions with Ca+2 ions, membrane phospholipids, and factor VIII. Alterations of this gene, including point mutations, insertions and deletions, cause factor IX deficiency, which is a recessive X-linked disorder, also called hemophilia B or Christmas disease. Alternative splicing results in multiple transcript variants encoding different isoforms that may undergo similar proteolytic processing. [provided by RefSeq, Sep 2015]

Uniprot Description

F9: Factor IX is a vitamin K-dependent plasma protein that participates in the intrinsic pathway of blood coagulation by converting factor X to its active form in the presence of Ca(2+) ions, phospholipids, and factor VIIIa. Defects in F9 are the cause of recessive X-linked hemophilia B (HEMB); also known as Christmas disease. Mutations in position 43 (Oxford-3, San Dimas) and 46 (Cambridge) prevents cleavage of the propeptide, mutation in position 93 (Alabama) probably fails to bind to cell membranes, mutation in position 191 (Chapel-Hill) or in position 226 (Nagoya OR Hilo) prevent cleavage of the activation peptide. Defects in F9 are the cause of thrombophilia due to factor IX defect (THPH8). A hemostatic disorder characterized by a tendency to thrombosis. Belongs to the peptidase S1 family.

Protein type: EC 3.4.21.22; Protease; Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: Xq27.1-q27.2

Cellular Component: endoplasmic reticulum lumen; extracellular region; extracellular space; Golgi lumen; plasma membrane

Molecular Function: calcium ion binding; endopeptidase activity; serine-type endopeptidase activity

Biological Process: blood coagulation; blood coagulation, extrinsic pathway; blood coagulation, intrinsic pathway; cellular protein metabolic process; ER to Golgi vesicle-mediated transport; peptidyl-glutamic acid carboxylation; post-translational protein modification; proteolysis; signal peptide processing; zymogen activation

Disease: Coumarin Resistance; Hemophilia B; Thrombophilia, X-linked, Due To Factor Ix Defect