Fish metallothionein (MT) ELISA Kit | MT elisa kit

Fish metallothionein, MT ELISA Kit

Cat. Num. AAA903939

• Reactivity: Fish
• Synonyms: metallothionein (MT); Fish metallothionein; MT ELISA Kit; Fish metallothionein (MT) ELISA kit; MT elisa kit

• Reactivity: Fish
• Specificity: This assay has high sensitivity and excellent specificity for detection of fish MT. No significant cross-reactivity or interference between fish MT and analogues was observed.
• Sequence Length: 53

• Samples: Serum, plasma
• Assay Type: Quantitative Competitive
• Detection Range: 25 pg/ml-400 pg/ml
• Sensitivity: < 12.5 pg/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for MT elisa kit

Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to MT. Standards or samples are added to the appropriate microtiter plate wells with Horseradish Peroxidase (HRP) conjugated MT. The competitive inhibition reaction is launched between with HRP-conjugated MT and MT in samples. A substrate solution is added to the wells and the color develops in opposite to the amount of MT in the sample. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 313471345
• NCBI GeneID: 923079
• UniProt Accession #: P9WK08; P0CI28; Q8VKQ2
• Molecular Weight: 5,719 Da
• NCBI Official Symbol: MT0196
• NCBI Protein Information: hypothetical protein
• UniProt Protein Name: Metallothionein
• UniProt Gene Name: mymT
• UniProt Synonym Gene Names: MT0196; MT; Cu(I)-binding metallothionein
• UniProt Entry Name: MYMT_MYCTO

Uniprot Description

Function: Metallothioneins are small proteins that have a high content of cysteine residues wich allow them to bind heavy metal ions through clusters of thiolate bonds. MymT binds up to seven ions of Cu+, with a preference for four to six Cu+ ions, in a solvent-shielded core. MymT protects M.tuberculosis from copper toxicity