Porcine Interleukin 1 Eta ELISA Kit | FIL1h elisa kit

Porcine Interleukin 1 Eta ELISA Kit

Cat. Num. AAA737979

• Gene Names: IL36B; FIL1; FIL1H; IL1F8; IL1H2; IL-1F8; IL-1H2; IL1-ETA; FIL1-(ETA); FILI-(ETA)
• Reactivity: Porcine
• Synonyms: Interleukin 1 Eta; Porcine Interleukin 1 Eta ELISA Kit; FIL1h elisa kit

• Reactivity: Porcine

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Detection Range: 50-1000pg/mL
• Sensitivity: 1.0pg/mL
• Intended Uses: This FIL1h ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Porcine FIL1h. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for FIL1h elisa kit

Principle of the Assay: FIL1h ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for FIL1h. Standards or samples are then added to the microtiter plate wells and FIL1h if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of FIL1h present in the sample, a standardized preparation of horseradish peroxidase (HRP) -conjugated polyclonal antibody, specific for FIL1h are added to each well to "sandwich" the FIL1h immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain FIL1h and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The FIL1h concentration in each sample is interpolated from this standard curve.

Product Categories/Family for FIL1h elisa kit

Immunology

NCBI and Uniprot Product Information

• NCBI GI #: 20070252
• NCBI GeneID: 27177
• NCBI Accession #: NP_055253.2
• NCBI GenBank Nucleotide #: NM_014438.4
• Molecular Weight: 17,702 Da
• NCBI Official Full Name: interleukin-36 beta isoform 1
• NCBI Official Synonym Full Names: interleukin 36, beta
• NCBI Official Symbol: IL36B
• NCBI Official Synonym Symbols: FIL1; FIL1H; IL1F8; IL1H2; IL-1F8; IL-1H2; IL1-ETA; FIL1-(ETA); FILI-(ETA)
• NCBI Protein Information: interleukin-36 beta; FIL1 eta; IL-1 eta; IL-1F8 (FIL1-eta); interleukin-1 eta; interleukin 1, eta; interleukin-1 homolog 2; Interleukin-1 Superfamily e; family of interleukin 1-eta; interleukin-1 family member 8; IL1F8 (Canonical product IL-1F8a); interleukin 1 family, member 8 (eta)
• UniProt Protein Name: Interleukin-36 beta
• UniProt Gene Name: IL36B
• UniProt Synonym Gene Names: IL1F8; IL1H2; IL-1 eta; IL-1F8; IL-1H2
• UniProt Entry Name: IL36B_HUMAN

NCBI Description

The protein encoded by this gene is a member of the interleukin 1 cytokine family. Protein structure modeling indicated that this cytokine may contain a 12-stranded beta-trefoil structure that is conserved between IL1A (IL-A alpha) and IL1B (IL-1 beta). This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. Two alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008]

Uniprot Description

IL36B: Stimulates production of interleukin-6 and interleukin-8 in synovial fibrobasts, articular chondrocytes and mature adipocytes. Induces expression of a number of antimicrobial peptides including beta-defensins 4 and 103 as well as a number of matrix metalloproteases. Belongs to the IL-1 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cytokine

Chromosomal Location of Human Ortholog: 2q14

Cellular Component: extracellular space; extracellular region

Molecular Function: interleukin-1 receptor binding; cytokine activity

Biological Process: positive regulation of T cell differentiation; cytokine and chemokine mediated signaling pathway; positive regulation of interleukin-6 production; innate immune response; immune response; inflammatory response