Human Apolipoprotein A-I ELISA Kit | APOA1 elisa kit

Human Apolipoprotein A-I ELISA Kit

Cat. Num. AAA766037

• Gene Names: APOA1; apo(a)
• Reactivity: Human
• Synonyms: Apolipoprotein A-I; Human Apolipoprotein A-I ELISA Kit; APOA1/Apo-A1/Alp-1/Brp-14/Ltw-1/Lvtw-1/Sep-1/Sep-2/apo-AI/Apo-AI/ApoA-I/apoA-I/Apolipoprotein A1/apolipoprotein A-I/MGC117399; APOA1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of APOA1. No significant cross-reactivity or interference between APOA1 and analogues was observed.
• Sequence Length: 267

• Samples: Serum, plasma, tissue homogenates and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 0.313-20ng/ml
• Sensitivity: 0.188ng/ml
• Intra-assay Precision: CV<8%
• Inter-assay Precision: CV<10%
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

()

Related Product Information for APOA1 elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Capture antibody was pre-coated onto 96-well plates. And the biotin conjugated antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of target can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 966751413
• NCBI GeneID: 335
• NCBI Accession #: NP_001304947.1
• NCBI GenBank Nucleotide #: NM_001318018.1
• Molecular Weight: 30,778 Da
• NCBI Official Full Name: apolipoprotein A-I isoform 1 preproprotein
• NCBI Official Synonym Full Names: apolipoprotein A-I
• NCBI Official Symbol: APOA1
• NCBI Official Synonym Symbols: apo(a)
• NCBI Protein Information: apolipoprotein A-I
• UniProt Protein Name: Apolipoprotein A-I
• Protein Family: Apolipoprotein
• UniProt Gene Name: APOA1
• UniProt Synonym Gene Names: Apo-AI; ApoA-I; ProapoA-I
• UniProt Entry Name: APOA1_HUMAN

NCBI Description

This gene encodes apolipoprotein A-I, which is the major protein component of high density lipoprotein (HDL) in plasma. The encoded preproprotein is proteolytically processed to generate the mature protein, which promotes cholesterol efflux from tissues to the liver for excretion, and is a cofactor for lecithin cholesterolacyltransferase (LCAT), an enzyme responsible for the formation of most plasma cholesteryl esters. This gene is closely linked with two other apolipoprotein genes on chromosome 11. Defects in this gene are associated with HDL deficiencies, including Tangier disease, and with systemic non-neuropathic amyloidosis. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein. [provided by RefSeq, Dec 2015]

Uniprot Description

APOA1: Participates in the reverse transport of cholesterol from tissues to the liver for excretion by promoting cholesterol efflux from tissues and by acting as a cofactor for the lecithin cholesterol acyltransferase (LCAT). As part of the SPAP complex, activates spermatozoa motility. Interacts with APOA1BP and CLU. Component of a sperm activating protein complex (SPAP), consisting of APOA1, an immunoglobulin heavy chain, an immunoglobulin light chain and albumin. Interacts with NDRG1. Major protein of plasma HDL, also found in chylomicrons. Synthesized in the liver and small intestine. The oxidized form at Met-110 and Met-136 is increased in individuals with increased risk for coronary artery disease, such as in carrier of the eNOSa/b genotype and exposure to cigarette smoking. It is also present in increased levels in aortic lesions relative to native ApoA-I and increased levels are seen with increasing severity of disease. Belongs to the apolipoprotein A1/A4/E family.

Protein type: Vesicle; Secreted; Secreted, signal peptide; Endoplasmic reticulum; Lipid-binding; Motility/polarity/chemotaxis; Cell development/differentiation

Chromosomal Location of Human Ortholog: 11q23-q24

Cellular Component: cell surface; chylomicron; cytoplasmic vesicle; cytosol; early endosome; endocytic vesicle; endoplasmic reticulum lumen; extracellular region; extracellular space; nucleus; plasma membrane

Molecular Function: apolipoprotein A-I receptor binding; apolipoprotein receptor binding; beta-amyloid binding; chemorepellent activity; cholesterol binding; cholesterol transporter activity; enzyme binding; high-density lipoprotein binding; identical protein binding; lipase inhibitor activity; phosphatidylcholine binding; phospholipid binding; phospholipid transporter activity; protein binding

Biological Process: adrenal gland development; axon regeneration in the peripheral nervous system; blood coagulation; blood vessel endothelial cell migration; cellular lipid metabolic process; cellular protein metabolic process; cholesterol biosynthetic process; cholesterol efflux; cholesterol homeostasis; cholesterol metabolic process; cholesterol transport; endothelial cell proliferation; fat-soluble vitamin metabolic process; G-protein coupled receptor protein signaling pathway; glucocorticoid metabolic process; integrin-mediated signaling pathway; lipoprotein biosynthetic process; lipoprotein metabolic process; negative chemotaxis; negative regulation of cytokine secretion during immune response; negative regulation of heterotypic cell-cell adhesion; negative regulation of inflammatory response; negative regulation of interleukin-1 beta secretion; negative regulation of lipase activity; neurite regeneration; organ regeneration; peptidyl-methionine modification; phosphatidylcholine biosynthetic process; phospholipid efflux; phospholipid homeostasis; phototransduction, visible light; platelet activation; platelet degranulation; positive regulation of fatty acid biosynthetic process; positive regulation of hydrolase activity; positive regulation of lipoprotein lipase activity; positive regulation of Rho protein signal transduction; positive regulation of stress fiber formation; protein amino acid oxidation; protein stabilization; receptor-mediated endocytosis; regulation of Cdc42 protein signal transduction; regulation of cholesterol absorption; regulation of protein amino acid phosphorylation; response to drug; response to estrogen stimulus; response to nutrient; retinoid metabolic process; reverse cholesterol transport; sequestering of lipid; transforming growth factor beta receptor signaling pathway; transmembrane transport; triacylglycerol catabolic process; vitamin metabolic process; vitamin transport

Disease: Amyloidosis, Familial Visceral; Hypoalphalipoproteinemia, Primary