Fibrinogen Gamma (FGg) Recombinant Protein | FGg recombinant protein

Recombinant Fibrinogen Gamma (FGg)

Cat. Num. AAA2009149

• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Fibrinogen Gamma (FGg); Recombinant Fibrinogen Gamma (FGg); FGg recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with N-terminal His-Tag, its sequence is listed below.; MGHHHHHHSGSEF-R IHDTTGKDCQ DIANKGAKES GLYFIRPLKA TQQFLVYCEI DGSGNGWTVL QKRLDGSVDF KKNWIQYKEG FGHLSPTGTT EFWLGNEKIH LISMQSTIPY ALRIQLKDWS GRTSTADYAM FRVGPESDKY RLTYAYFIGG DAGDAFDGYD FGDDPSDKFF TSHNGMHFST WDNDNDKFEG NCAEQDGSGW WMNKCHAGHL NGVYYQGGTY SKSSTPNGYD NGIIWATWKT RWYSMKETTM KIIPFNRLSI GDGQQHHMG
• Sequence Length: 437

• Applicable Applications for FGg recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Rattus norvegicus (Rat)
• Expression System: Prokaryotic expression
• Residues: Arg170~Gly429 (Accession # P02680) with N-terminal His-Tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

NCBI and Uniprot Product Information

• NCBI GI #: 61098186
• NCBI GeneID: 24367
• NCBI Accession #: NP_036691.2
• NCBI GenBank Nucleotide #: NM_012559.2
• UniProt Accession #: P02680; Q68FY3
• Molecular Weight: 31.1kDa
• NCBI Official Full Name: fibrinogen gamma chain
• NCBI Official Synonym Full Names: fibrinogen gamma chain
• NCBI Official Symbol: Fgg
• NCBI Protein Information: fibrinogen gamma chain; fibrinogen, gamma polypeptide
• UniProt Protein Name: Fibrinogen gamma chain
• Protein Family: Fibrinogen
• UniProt Gene Name: Fgg
• UniProt Entry Name: FIBG_RAT

NCBI Description

combines with alpha and beta polypeptides to form the mature fibrinogen molecule; plays a role in inflammatory response [RGD, Feb 2006]

Uniprot Description

FGG: Fibrinogen has a double function: yielding monomers that polymerize into fibrin and acting as a cofactor in platelet aggregation. Defects in FGG are a cause of congenital afibrinogenemia (CAFBN). This rare autosomal recessive disorder is characterized by bleeding that varies from mild to severe and by complete absence or extremely low levels of plasma and platelet fibrinogen. Patients with congenital fibrinogen abnormalities can manifest different clinical pictures. Some cases are clinically silent, some show a tendency toward bleeding and some show a predisposition for thrombosis with or without bleeding. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Secreted

Cellular Component: extracellular space; cell surface; cytoplasm; fibrinogen complex; cell cortex; external side of plasma membrane

Molecular Function: protein binding, bridging; metal ion binding; cell adhesion molecule binding; structural molecule activity; receptor binding

Biological Process: protein polymerization; cellular protein complex assembly; positive regulation of heterotypic cell-cell adhesion; positive regulation of protein secretion; cell-matrix adhesion; positive regulation of vasoconstriction; signal transduction; inflammatory response; response to calcium ion; positive regulation of exocytosis