Cartilage Oligomeric Matrix Protein (COMP) Recombinant Protein | COMP recombinant protein

Recombinant Cartilage Oligomeric Matrix Protein (COMP)

Cat. Num. AAA2011955

• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Cartilage Oligomeric Matrix Protein (COMP); Recombinant Cartilage Oligomeric Matrix Protein (COMP); COMP recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with N-terminal His-Tag, its sequence is listed below.; MGHHHHHHSGSEF- CHEKAD CILERDGSRS CVCAVGWAGN GLLCGRDTDL DGFPDEKLRC SERQCRKDNC VTVPNSGQED VDRDRIGDAC DPDADGDGVP NEQDNCPLVR NPDQRNSDKD KWGDACDNCR SQKNDDQKDT DRDGQGDACD DDIDGDRIRN VADNCPRVPN FDQSDSDGDG VGDACDNCPQ KDNPDQRDVD HDF
• Sequence Length: 755

• Applicable Applications for COMP recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Rattus norvegicus (Rat)
• Expression System: Prokaryotic expression
• Residues: Cys235~Phe423 (Accession # P35444) with N-terminal His-Tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 6978679
• NCBI GeneID: 25304
• NCBI Accession #: NP_036966.1
• NCBI GenBank Nucleotide #: NM_012834.1
• UniProt Accession #: P35444
• Molecular Weight: 22.3kDa
• NCBI Official Full Name: cartilage oligomeric matrix protein
• NCBI Official Synonym Full Names: cartilage oligomeric matrix protein
• NCBI Official Symbol: Comp
• NCBI Protein Information: cartilage oligomeric matrix protein
• UniProt Protein Name: Cartilage oligomeric matrix protein
• Protein Family: Cartilage oligomeric matrix protein
• UniProt Gene Name: Comp
• UniProt Synonym Gene Names: COMP
• UniProt Entry Name: COMP_RAT

NCBI Description

extracellular matrix protein that interacts with fibronectin and may play a role in extracellular matrix assembly and molecular interactions [RGD, Feb 2006]

Uniprot Description

Function: May play a role in the structural integrity of cartilage via its interaction with other extracellular matrix proteins such as the collagens and fibronectin. Can mediate the interaction of chondrocytes with the cartilage extracellular matrix through interaction with cell surface integrin receptors. Could play a role in the pathogenesis of osteoarthritis. Potent suppressor of apoptosis in both primary chondrocytes and transformed cells. Suppresses apoptosis by blocking the activation of caspase-3 and by inducing the IAP family of survival proteins (BIRC3, BIRC2, BIRC5 and XIAP)

By similarity. Essential for maintaining a vascular smooth muscle cells (VSMCs) contractile/differentiated phenotype under physiological and pathological stimuli. Maintains this phenotype of VSMCs by interacting with ITGA7. Ref.2

Cofactor: Binds 11-14 calcium ions per subunit

By similarity.

Subunit structure: Pentamer; disulfide-linked. Exists in a more compact conformation in the presence of calcium and shows a more extended conformation in the absence of calcium. Interacts with ITGB3, ITGA5 and FN1. Binding to FN1 requires the presence of divalent cations (Ca2+, Mg2+ or Mn2+). The greatest amount of binding is seen in the presence of Mn2+. Interacts with MATN1, MATN3, MATN4 and ACAN. Binds heparin, heparan sulfate and chondroitin sulfate. EDTA dimishes significantly its binding to ACAN and abolishes its binding to MATN3, MATN4 and chondroitin sulfate. Interacts with collagen I, II and IX and interaction with these collagens is dependent on the presence of zinc ions

By similarity. Interacts with ADAMTS12

By similarity. Interacts with ITGA7. Ref.2

Subcellular location: Secreted › extracellular space › extracellular matrix

By similarity.

Tissue specificity: Expressed in differentiated and de-differentiated vascular smooth muscle cells (VSMCs) (at protein level). Ref.2

Domain: The cell attachment motif mediates the attachment to chondrocytes. It mediates the induction of both the IAP family of survival proteins and the antiapoptotic response

By similarity.The TSP C-terminal domain mediates interaction with FN1 and ACAN

By similarity.Each of the eight TSP type-3 repeats binds two calcium ions. The TSP C-terminal domain binds three calcium ions

By similarity.

Sequence similarities: Belongs to the thrombospondin family.Contains 4 EGF-like domains.Contains 1 TSP C-terminal (TSPC) domain.Contains 8 TSP type-3 repeats.