Bovine Fibroblast Growth Factor 9 ELISA Kit | FGF9 elisa kit

Bovine Fibroblast Growth Factor 9 ELISA Kit

Cat. Num. AAA742153

• Gene Names: FGF9; GAF; FGF-9; SYNS3; HBFG-9; HBGF-9
• Reactivity: Bovine
• Synonyms: Fibroblast Growth Factor 9; Bovine Fibroblast Growth Factor 9 ELISA Kit; FGF9 elisa kit

• Reactivity: Bovine

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Detection Range: 250-5000pg/ml
• Sensitivity: 1.0pg/ml
• Intended Uses: This FGF9 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Bovine FGF9. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for FGF9 elisa kit

Principle of the Assay: FGF9 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-FGF9 antibody and an FGF9-HRP conjugate. The assay sample and buffer are incubated together with FGF9-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the FGF9 concentration since FGF9 from samples and FGF9-HRP conjugate compete for the anti-FGF9 antibody binding site. Since the number of sites is limited, as more sites are occupied by FGF9 from the sample, fewer sites are left to bind FGF9-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The FGF9 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for FGF9 elisa kit

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 391719
• NCBI GeneID: 2254
• Molecular Weight: 23,441 Da
• NCBI Official Full Name: fibroblast growth factor 9
• NCBI Official Synonym Full Names: fibroblast growth factor 9
• NCBI Official Symbol: FGF9
• NCBI Official Synonym Symbols: GAF; FGF-9; SYNS3; HBFG-9; HBGF-9
• NCBI Protein Information: fibroblast growth factor 9; heparin-binding growth factor 9; fibroblast growth factor 9 (glia-activating factor)
• UniProt Protein Name: Fibroblast growth factor 9
• Protein Family: Fibroblast growth factor
• UniProt Gene Name: FGF9
• UniProt Synonym Gene Names: FGF-9; GAF; HBGF-9
• UniProt Entry Name: FGF9_HUMAN

NCBI Description

The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This protein was isolated as a secreted factor that exhibits a growth-stimulating effect on cultured glial cells. In nervous system, this protein is produced mainly by neurons and may be important for glial cell development. Expression of the mouse homolog of this gene was found to be dependent on Sonic hedgehog (Shh) signaling. Mice lacking the homolog gene displayed a male-to-female sex reversal phenotype, which suggested a role in testicular embryogenesis. [provided by RefSeq, Jul 2008]

Uniprot Description

FGF9: Plays an important role in the regulation of embryonic development, cell proliferation, cell differentiation and cell migration. May have a role in glial cell growth and differentiation during development, gliosis during repair and regeneration of brain tissue after damage, differentiation and survival of neuronal cells, and growth stimulation of glial tumors. Defects in FGF9 are the cause of multiple synostoses syndrome type 3 (SYNS3). Multiple synostoses syndrome is an autosomal dominant condition characterized by progressive joint fusions of the fingers, wrists, ankles and cervical spine, characteristic facies and progressive conductive deafness. Belongs to the heparin-binding growth factors family.

Protein type: Cytokine

Chromosomal Location of Human Ortholog: 13q11-q12

Cellular Component: extracellular space; cytoplasm; extracellular region; basement membrane

Molecular Function: heparin binding; growth factor activity; fibroblast growth factor receptor binding

Biological Process: negative regulation of Wnt receptor signaling pathway; nerve growth factor receptor signaling pathway; embryonic skeletal development; negative regulation of transcription from RNA polymerase II promoter; signal transduction; positive regulation of vascular endothelial growth factor receptor signaling pathway; positive regulation of activin receptor signaling pathway; substantia nigra development; positive regulation of MAPKKK cascade; cell-cell signaling; protein import into nucleus; male sex determination; positive regulation of cell proliferation; positive regulation of mesenchymal cell proliferation; chondrocyte differentiation; positive regulation of smoothened signaling pathway; angiogenesis; embryonic gut development; regulation of timing of cell differentiation; positive regulation of cardiac muscle cell proliferation; embryonic limb morphogenesis; epidermal growth factor receptor signaling pathway; inner ear morphogenesis; fibroblast growth factor receptor signaling pathway; phosphoinositide-mediated signaling; male gonad development; osteoblast differentiation; positive regulation of cell division; insulin receptor signaling pathway; innate immune response; positive regulation of epithelial cell proliferation

Disease: Multiple Synostoses Syndrome 3