Fibroblast Growth Factor 8 Recombinant Protein | FGF8 recombinant protein

Fibroblast Growth Factor 8, Recombinant, Human (FGF8)

Cat. Num. AAA650117

• Gene Names: FGF8; AIGF; KAL6; FGF-8; HBGF-8; MGC149376
• Purity: Molecular Biology Grade; 95% by SDS-PAGE and HPLC. Endotoxin: 0.1ng/ug (1EU/ug).
• Synonyms: Fibroblast Growth Factor 8; Recombinant; Human (FGF8); FGF8 recombinant protein

• Host: Recombinant, Human (E Coli)
• Specificity: Biological Activity: The ED50 as determined by the dose-dependent stimulation of thymidine uptake by BaF3 cells expressing FGF-receptors is 0.5ng/ml, corresponding to a speci c activity of 2 x 10e6u/mg
• Purity/Purification: Molecular Biology Grade; 95% by SDS-PAGE and HPLC. Endotoxin: 0.1ng/ug (1EU/ug).
• Form/Format: Supplied as a lyophilized powder from 10mM Tris, pH 8.0, 75mM sodium chloride. Reconstitute in 10 mM Tris, pH 8.0.

• Country of Origin: USA
• Preparation and Storage: Lyophilized powder may be stored at -20 degree C. Reconstitute with 10mM Tris, pH 8.0, 75mM sodium chloride, aliquot and store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for FGF8 recombinant protein

Fibroblast Growth Factor-8 (FGF-8b) is a heparin binding growth factor belonging to the FGF family. Proteins of this family play a central role during prenatal development and postnatal growth and regeneration of a variety of tissues, by promoting cellular proliferation and differentiation. There are 4 known alternate spliced forms of FGF8: FGF8A, FGF-8B, FGF-8E and FGF-8F. The human and murine FGF8 A and B are identical. Human and murine FGF8 E and F are 98% identical. FGF-8 targets mammary carcinoma cells and other cells expressing the FGF receptors. Recombinant human FGF-8b is a 22.4kD protein consisting of 193 amino acid residues.

Product Categories/Family for FGF8 recombinant protein

Growth Factors; Cytokines; Growth Factors-FGF

NCBI and Uniprot Product Information

• NCBI GI #: 1143262
• NCBI GeneID: 2253
• UniProt Accession #: P55075; Q14915; Q15766; A1A514
• Molecular Weight: 22.4kD
• NCBI Official Full Name: fibroblast growth factor 8
• NCBI Official Synonym Full Names: fibroblast growth factor 8 (androgen-induced)
• NCBI Official Symbol: FGF8
• NCBI Official Synonym Symbols: AIGF; KAL6; FGF-8; HBGF-8; MGC149376
• NCBI Protein Information: fibroblast growth factor 8; OTTHUMP00000020348; OTTHUMP00000020349; OTTHUMP00000020350; OTTHUMP00000020351; androgen-induced growth factor; heparin-binding growth factor 8
• UniProt Protein Name: Fibroblast growth factor 8
• Protein Family: Fibroblast growth factor
• UniProt Gene Name: FGF8
• UniProt Synonym Gene Names: AIGF
• UniProt Entry Name: FGF8_HUMAN

NCBI Description

The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This protein is known to be a factor that supports androgen and anchorage independent growth of mammary tumor cells. Overexpression of this gene has been shown to increase tumor growth and angiogensis. The adult expression of this gene is restricted to testes and ovaries. Temporal and spatial pattern of this gene expression suggests its function as an embryonic epithelial factor. Studies of the mouse and chick homologs revealed roles in midbrain and limb development, organogenesis, embryo gastrulation and left-right axis determination. The alternative splicing of this gene results in four transcript variants. [provided by RefSeq]

Uniprot Description

Function: Stimulates growth of the cells in an autocrine manner. Mediates hormonal action on the growth of cancer cells.

Subcellular location: Secreted.

Developmental stage: In adults expression is restricted to the gonads.

Involvement in disease: Defects in FGF8 are the cause of Kallmann syndrome type 6 (KAL6) [

MIM:612702]. Kallmann syndrome is a disorder that associates hypogonadotropic hypogonadism and anosmia. Anosmia or hyposmia is related to the absence or hypoplasia of the olfactory bulbs and tracts. Hypogonadism is due to deficiency in gonadotropin-releasing hormone and probably results from a failure of embryonic migration of gonadotropin-releasing hormone-synthesizing neurons. In some patients other developmental anomalies can be present, which include renal agenesis, cleft lip and/or palate, selective tooth agenesis, and bimanual synkinesis. In some cases anosmia may be absent or inconspicuous. Ref.9Defects in FGF8 are a cause of idiopathic hypogonadotropic hypogonadism (IHH) [

MIM:146110]. IHH is defined as a deficiency of the pituitary secretion of follicle-stimulating hormone and luteinizing hormone, which results in the impairment of pubertal maturation and of reproductive function. Ref.9

Sequence similarities: Belongs to the heparin-binding growth factors family.