Fibroblast Growth Factor-8 Active Protein | FGF8 active protein

Recombinant Mouse Fibroblast Growth Factor-8, 194 a.a.

Cat. Num. AAA140958

• Gene Names: Fgf8; Aigf; Fgf-8
• Purity: Greater than 97.0% as determined by analysis by SDS-PAGE.
• Synonyms: Fibroblast Growth Factor-8; Recombinant Mouse Fibroblast Growth Factor-8; 194 a.a.; FGF 8 Mouse; Fibroblast Growth Factor-8 Mouse Recombinant; FGF8B; FGF-8B; FGF8-B; KAL6; HBGF-8; HBGF8; AIGF; MGC149376; fibroblast growth factor 8; FGF8 active protein

• Purity/Purification: Greater than 97.0% as determined by analysis by SDS-PAGE.
• Form/Format: Lyophilized from a concentrated (1mg/ml) solution containing 5mM Na3PO4 and 50 mM NaCl, pH 7.5.; Sterile Filtered White lyophilized (freeze-dried) powder.
• Sequence: MQVTVQSSPN FTQHVREQSL VTDQLSRRLI RTYQLYSRTS GKHVQVLANK RINAMAEDGDPFAKLIVETD TFGSRVRVRG AETGLYICMN KKGKLIAKSN GKGKDCVFTE IVLENNYTAL QNAKYEGWYM AFTRKGRPRK GSKTRQHQRE VHFMKRLPRG HHTTEQSLRF EFLNYPPFTR SLRGSQRTWA PEPR
• Sequence Length: 215

• Source: Escherichia Coli
• Biological Activity: The ED₅₀, as determined by its ability to induce proliferation of mouse 3T3 fibroblasts, is 5-7ng/ml, corresponding to a specific activity of 2 x 10⁵units/mg
• Solubility: It is recommended to reconstitute the lyophilized FGF 8 in sterile 18M-cm H2O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions.
• Preparation and Storage: Lyophilized FGF 8 although stable at room temperature for 3 weeks, should be stored desiccated below -18?C. Upon reconstitution FGF 8 should be stored at 4?C between 2-7 days and for future use below -18?C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.

Related Product Information for FGF8 active protein

Introduction: FGF8 is part of the fibroblast growth factor family. FGF family members have wide mitogenic and cell survival activities, and participate in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. FGF8 supports androgen and anchorage independent growth of mammary tumor cells. FGF8 over expression increases tumor growth and angiogensis. The adult expression of FGF-8 gene is restricted to testes and ovaries. FGF8 functions as an embryonic epithelial factor. FGF8 takes part in midbrain and limb development, organogenesis, embryo gastrulation and left-right axis determination.

Description: FGF 8 Mouse Recombinant produced in E Coli is a non-glycosylated polypeptide chain containing 194 amino acids and having a total molecular mass of 22.5kDa.

Product Categories/Family for FGF8 active protein

Cytokines and Growth Factors

NCBI and Uniprot Product Information

• NCBI GI #: 261599076
• NCBI GeneID: 14179
• NCBI Accession #: NP_001159834.1
• NCBI GenBank Nucleotide #: NM_001166362.1
• UniProt Accession #: P37237
• Molecular Weight: 23,522 Da
• NCBI Official Full Name: fibroblast growth factor 8 isoform 3
• NCBI Official Synonym Full Names: fibroblast growth factor 8
• NCBI Official Symbol: Fgf8
• NCBI Official Synonym Symbols: Aigf; Fgf-8
• NCBI Protein Information: fibroblast growth factor 8
• UniProt Protein Name: Fibroblast growth factor 8
• UniProt Gene Name: Fgf8
• UniProt Synonym Gene Names: Aigf; FGF-8; AIGF; HBGF-8
• UniProt Entry Name: FGF8_MOUSE

Uniprot Description

FGF8: Plays an important role in the regulation of embryonic development, cell proliferation, cell differentiation and cell migration. Required for normal brain, eye, ear and limb development during embryogenesis. Required for normal development of the gonadotropin-releasing hormone (GnRH) neuronal system. Defects in FGF8 are the cause of Kallmann syndrome type 6 (KAL6). Kallmann syndrome is a disorder that associates hypogonadotropic hypogonadism and anosmia. Anosmia or hyposmia is related to the absence or hypoplasia of the olfactory bulbs and tracts. Hypogonadism is due to deficiency in gonadotropin-releasing hormone and probably results from a failure of embryonic migration of gonadotropin-releasing hormone- synthesizing neurons. In some patients other developmental anomalies can be present, which include renal agenesis, cleft lip and/or palate, selective tooth agenesis, and bimanual synkinesis. In some cases anosmia may be absent or inconspicuous. Defects in FGF8 are a cause of idiopathic hypogonadotropic hypogonadism (IHH). IHH is defined as a deficiency of the pituitary secretion of follicle-stimulating hormone and luteinizing hormone, which results in the impairment of pubertal maturation and of reproductive function. Belongs to the heparin-binding growth factors family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Cytokine; Secreted; Secreted, signal peptide

Cellular Component: external side of plasma membrane

Molecular Function: chemoattractant activity

Biological Process: adrenocorticotropin hormone secreting cell differentiation; apoptosis; blood vessel remodeling; BMP signaling pathway; cell fate commitment; cell proliferation in forebrain; central nervous system neuron development; determination of left/right symmetry; dorsal/ventral axon guidance; embryonic development ending in birth or egg hatching; embryonic heart tube development; embryonic hindlimb morphogenesis; fibroblast growth factor receptor signaling pathway; forebrain dorsal/ventral pattern formation; forebrain morphogenesis; forebrain neuron development; generation of neurons; gonad development; heart development; heart looping; heart morphogenesis; induction of an organ; inner ear morphogenesis; kidney development; limb morphogenesis; lung development; male genitalia development; MAPKKK cascade; mesodermal cell migration; midbrain-hindbrain boundary development; motor axon guidance; negative regulation of apoptosis; negative regulation of cardiac muscle development; negative regulation of neuron apoptosis; neural plate morphogenesis; otic vesicle formation; pallium development; patterning of blood vessels; pharyngeal system development; positive regulation of cell proliferation; positive regulation of mitosis; positive regulation of organ growth; regulation of odontogenesis of dentine-containing teeth; response to oxidative stress; subpallium development; telencephalon development; thyroid gland development; thyroid stimulating hormone secreting cell differentiation; ureteric bud branching; Wnt receptor signaling pathway through beta-catenin