Fibroblast Growth Factor 1, Acidic (FGF1) Recombinant Protein | FGF1 recombinant protein

Recombinant Fibroblast Growth Factor 1, Acidic (FGF1)

Cat. Num. AAA2011708

• Gene Names: Fgf1; FGF-1; HBGF1; HBGF-1
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Fibroblast Growth Factor 1; Acidic (FGF1); Recombinant Fibroblast Growth Factor 1; FGF1 recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and GST-tag, its sequence is listed below.; MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR GSTAIGMKET AAAKFERQHM DSPDLGTLEV LFQGPLGSEF-FNLPL GNYKKPKLLY CSNGGHFLRI LPDGTVDGTR DRSDQHIQLQ LSAESAGEVY IKGTETGQYL AMDTEGLLYG SQTPNEECLF LERLEENHYN TYTSKKHAEK NWFVGLKKNG SCKRGPRTHY GQKAILFLPLPVSSD
• Sequence Length: 155

• Applicable Applications for FGF1 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Rattus norvegicus (Rat)
• Expression System: Prokaryotic expression
• Residues: Phe16~Asp155 (Accession # P61149) with two N-terminal Tags, His-tag and GST-tag
• Subcellular Location: Secreted. Cytoplasm, cell cortex. Cytosol. Nucleus
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Predicted Isoelectric Point: 6.4
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 6978839
• NCBI GeneID: 25317
• NCBI Accession #: NP_036978.1
• NCBI GenBank Nucleotide #: NM_012846.1
• UniProt Accession #: P61149; P10935
• Molecular Weight: 47.8kDa
• NCBI Official Full Name: fibroblast growth factor 1
• NCBI Official Synonym Full Names: fibroblast growth factor 1 (acidic)
• NCBI Official Symbol: Fgf1
• NCBI Official Synonym Symbols: FGF-1; HBGF1; HBGF-1
• NCBI Protein Information: fibroblast growth factor 1; aFGF; acidic fibroblast growth factor; heparin-binding growth factor 1; Fibroblast growth factor 1 (heparin binding)
• UniProt Protein Name: Fibroblast growth factor 1
• Protein Family: Fibroblast growth factor
• UniProt Gene Name: Fgf1
• UniProt Synonym Gene Names: Fgf-1; Fgfa; FGF-1; aFGF; HBGF-1
• UniProt Entry Name: FGF1_RAT

NCBI Description

may play a role in neurite outgrowth; may regulate cell differentiation in the nervous system; may act in synergy with fibronectin to enhance neuronal cell adhesion [RGD, Feb 2006]

Uniprot Description

FGF1: Plays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. Functions as potent mitogen in vitro. Monomer. Homodimer. Interacts with FGFR1, FGFR2, FGFR3 and FGFR4. Affinity between fibroblast growth factors (FGFs) and their receptors is increased by heparan sulfate glycosaminoglycans that function as coreceptors. Found in a complex with FGFBP1, FGF1 and FGF2. Interacts with FGFBP1. Part of a Cu(2+)-dependent multiprotein aggregate containing FGF1, S100A13 and SYT1. Interacts with SYT1. Interacts with S100A13. Belongs to the heparin-binding growth factors family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell development/differentiation; Motility/polarity/chemotaxis; Cytokine

Cellular Component: nucleoplasm; extracellular space; proteinaceous extracellular matrix; cytoplasm; nucleolus; extracellular region; cytosol; nucleus

Molecular Function: heparin binding; protein binding; growth factor activity; Hsp70 protein binding; receptor binding; fibroblast growth factor receptor binding

Biological Process: fibroblast growth factor receptor signaling pathway; multicellular organismal development; positive regulation of transcription, DNA-dependent; positive regulation of cholesterol biosynthetic process; cardiac muscle cell proliferation; positive regulation of MAP kinase activity; cell proliferation; positive regulation of angiogenesis; induction of an organ; positive regulation of cell division; positive regulation of cell proliferation; positive regulation of transcription from RNA polymerase II promoter; angiogenesis; positive regulation of protein amino acid phosphorylation; cell differentiation; positive regulation of epithelial cell proliferation; positive regulation of cell migration; lung development