Rat Fibroblast Growth Factor-10 ELISA Kit | FGF-10 elisa kit

Rat Fibroblast Growth Factor-10 ELISA Kit

Cat. Num. AAA761062

• Reactivity: Rat
• Synonyms: Fibroblast Growth Factor-10; Rat Fibroblast Growth Factor-10 ELISA Kit; FGF10 (Fibroblast growth factor 10)/KGF-2/FGF-10/fibroblast growth factor 10/Keratinocyte growth factor 2/produced by fibroblasts of urinary bladder lamina propria; FGF-10 elisa kit

• Reactivity: Rat
• Sequence Length: 208

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 15.625-1000pg/ml
• Sensitivity: 9.375pg/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for FGF-10 elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. anti-Rat FGF-10 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Rat FGF-10 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Rat FGF-10 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Rat FGF-10 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 4758360
• NCBI GeneID: 2255
• NCBI Accession #: NP_004456.1
• NCBI GenBank Nucleotide #: NM_004465.1
• Molecular Weight: 23,436 Da
• NCBI Official Full Name: fibroblast growth factor 10
• NCBI Official Synonym Full Names: fibroblast growth factor 10
• NCBI Official Symbol: FGF10
• NCBI Protein Information: fibroblast growth factor 10
• UniProt Protein Name: Fibroblast growth factor 10
• UniProt Gene Name: FGF10
• UniProt Synonym Gene Names: FGF-10
• UniProt Entry Name: FGF10_HUMAN

NCBI Description

The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This protein exhibits mitogenic activity for keratinizing epidermal cells, but essentially no activity for fibroblasts, which is similar to the biological activity of FGF7. Studies of the mouse homolog of suggested that this gene is required for embryonic epidermal morphogenesis including brain development, lung morphogenesis, and initiation of lim bud formation. This gene is also implicated to be a primary factor in the process of wound healing. [provided by RefSeq, Jul 2008]

Uniprot Description

FGF10: Plays an important role in the regulation of embryonic development, cell proliferation and cell differentiation. Required for normal branching morphogenesis. May play a role in wound healing. Interacts with FGFR1 and FGFR2. Interacts with FGFBP1. Belongs to the heparin-binding growth factors family.

Protein type: Cytokine; Secreted, signal peptide; Secreted; Motility/polarity/chemotaxis; Cell development/differentiation

Chromosomal Location of Human Ortholog: 5p13-p12

Cellular Component: cell surface; extracellular matrix; extracellular region; extracellular space; nucleus; plasma membrane

Molecular Function: chemoattractant activity; fibroblast growth factor receptor binding; growth factor activity; heparin binding; protein binding; type 2 fibroblast growth factor receptor binding

Biological Process: actin cytoskeleton reorganization; activation of MAPK activity; activation of MAPKK activity; angiogenesis; axon guidance; blood vessel remodeling; branching morphogenesis of a tube; determination of left/right symmetry; embryonic camera-type eye development; embryonic digestive tract morphogenesis; embryonic genitalia morphogenesis; embryonic pattern specification; epidermal growth factor receptor signaling pathway; epithelial cell proliferation; establishment of mitotic spindle orientation; female genitalia morphogenesis; fibroblast growth factor receptor signaling pathway; G1/S-specific positive regulation of cyclin-dependent protein kinase activity; hair follicle morphogenesis; induction of an organ; induction of positive chemotaxis; innate immune response; insulin receptor signaling pathway; keratinocyte proliferation; lacrimal gland development; limb bud formation; male genitalia morphogenesis; MAPKKK cascade; mesonephros development; metanephros development; muscle cell fate commitment; negative regulation of cell differentiation; negative regulation of cell proliferation; nerve growth factor receptor signaling pathway; odontogenesis of dentine-containing teeth; otic vesicle formation; pancreas development; phosphoinositide-mediated signaling; pituitary gland development; positive chemotaxis; positive regulation of ATPase activity; positive regulation of DNA repair; positive regulation of DNA replication; positive regulation of epithelial cell proliferation; positive regulation of epithelial cell proliferation involved in wound healing; positive regulation of fibroblast proliferation; positive regulation of keratinocyte migration; positive regulation of lymphocyte proliferation; positive regulation of MAPKKK cascade; positive regulation of mitotic cell cycle; positive regulation of Notch signaling pathway; positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of Ras protein signal transduction; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; positive regulation of urothelial cell proliferation; positive regulation of vascular endothelial growth factor receptor signaling pathway; radial glial cell differentiation; Ras protein signal transduction; regulation of activin receptor signaling pathway; regulation of saliva secretion; regulation of smoothened signaling pathway; response to estradiol stimulus; response to lipopolysaccharide; salivary gland development; small GTPase mediated signal transduction; smooth muscle cell differentiation; somatic stem cell maintenance; spleen development; thymus development; thyroid gland development; tissue regeneration; urothelial cell proliferation; vascular endothelial growth factor receptor signaling pathway; white fat cell differentiation; wound healing

Disease: Aplasia Of Lacrimal And Salivary Glands; Lacrimoauriculodentodigital Syndrome