CPOX recombinant protein

CPOX, 111-454aa, Human, His tag, E Coli

Cat. Num. AAA205206

• Gene Names: CPOX; CPO; CPX; HCP
• Applications: SDS-Page
• Purity: > 95% by SDS-PAGE
• Synonyms: CPOX; 111-454aa; Human; His tag; E Coli; Coproporphyrinogen-III oxidase mitochondrial precursor; Coproporphyrinogen-III oxidase; mitochondrial precursor; CPO; CPX; HCP; CPOX recombinant protein

• Host: E Coli
• Purity/Purification: > 95% by SDS-PAGE
• Form/Format: Liquid. In 20mM Tris-HCl Buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol, 1mM DTT
• Concentration: 1 mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MGSSHHHHHH SSGLVPRGSH MGSTSLGRPE EEEDELAHRC SSFMAPPVTD LGELRRRPGD MKTKMELLIL ETQAQVCQAL AQVDGGANFS VDRWERKEGG GGISCVLQDG CVFEKAGVSI SVVHGNLSEE AAKQMRSRGK VLKTKDGKLP FCAMGVSSVI HPKNPHAPTI HFNYRYFEVE EADGNKQWWF GGGCDLTPTY LNQEDAVHFH RTLKEACDQH GPDLYPKFKK WCDDYFFIAH RGERRGIGGI FFDDLDSPSK EEVFRFVQSC ARAVVPSYIP LVKKHCDDSF TPQEKLWQQL RRGRYVEFNL LYDRGTKFGL FTPGSRIESI LMSLPLTARW EYMHSPSENS KEAEILEVLR HPRDWVR
• Sequence Length: 454

• Applicable Applications for CPOX recombinant protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for CPOX recombinant protein

Coproporphyrinogen-III oxidase, mitochondrial precursor, also known as CPOX, is a 454 amino acid mitochondrial enzyme that is localized to the inner membrane space of erythrocytes. It participates in the sixth step of heme biosynthesis by catalyzing the formation of protoporphyrinogen IX from coproporphyrinogen III. Mutations in human CPOX gene predict the clinical outcome of the disease, with either hepatic hereditary coproporphyria or hematological manifestations of erythropoietic harderoporphyria. Recombinant human CPOX protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography techniques.

Product Categories/Family for CPOX recombinant protein

Metabolism

References

Martasek P., et al. (1994) Hum Mol Genet. 3:477-480; Lamoril J., et al. (1995) Hum Mol Genet. 4:275-278.;

NCBI and Uniprot Product Information

• NCBI GI #: 41393599
• NCBI GeneID: 1371
• NCBI Accession #: NP_000088
• NCBI GenBank Nucleotide #: NM_000097.5
• UniProt Accession #: P36551; Q14060; Q53F08; Q8IZ45; Q96AF3; A8K275; B4DSD5
• Molecular Weight: 41.6 kDa (367aa) confirmed by MALDI-TOF
• NCBI Official Full Name: oxygen-dependent coproporphyrinogen-III oxidase, mitochondrial
• NCBI Official Synonym Full Names: coproporphyrinogen oxidase
• NCBI Official Symbol: CPOX
• NCBI Official Synonym Symbols: CPO; CPX; HCP
• NCBI Protein Information: oxygen-dependent coproporphyrinogen-III oxidase, mitochondrial
• UniProt Protein Name: Oxygen-dependent coproporphyrinogen-III oxidase, mitochondrial
• Protein Family: Oxygen-dependent coproporphyrinogen-III oxidase
• UniProt Gene Name: CPOX
• UniProt Synonym Gene Names: CPO; CPX; COX; Coprogen oxidase; Coproporphyrinogenase

NCBI Description

The protein encoded by this gene is the sixth enzyme of the heme biosynthetic pathway. The encoded enzyme is soluble and found in the intermembrane space of mitochondria. This enzyme catalyzes the stepwise oxidative decarboxylation of coproporphyrinogen III to protoporphyrinogen IX, a precursor of heme. Defects in this gene are a cause of hereditary coproporphyria (HCP).[provided by RefSeq, Oct 2009]

Uniprot Description

Involved in the heme biosynthesis. Catalyzes the aerobic oxidative decarboxylation of propionate groups of rings A and B of coproporphyrinogen-III to yield the vinyl groups in protoporphyrinogen-IX.