Typical Testing Data/Standard Curve (for reference only)
Bovine Fibroblast growth factor-10 ELISA Kit | FGF-10 elisa kit
Bovine Fibroblast growth factor-10 ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
INTENDED USE This FGF-10 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Bovine FGF-10. This ELISA kit for research use only!
PRINCIPLE OF THE ASSAY FGF-10 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-FGF-10 antibody and an FGF-10-HRP conjugate. The assay sample and buffer are incubated together with FGF-10-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the FGF-10 concentration since FGF-10 from samples and FGF-10-HRP conjugate compete for the anti-FGF-10 antibody binding site. Since the number of sites is limited, as more sites are occupied by FGF-10 from the sample, fewer sites are left to bind FGF-10-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The FGF-10 concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This protein exhibits mitogenic activity for keratinizing epidermal cells, but essentially no activity for fibroblasts, which is similar to the biological activity of FGF7. Studies of the mouse homolog of suggested that this gene is required for embryonic epidermal morphogenesis including brain development, lung morphogenesis, and initiation of lim bud formation. This gene is also implicated to be a primary factor in the process of wound healing. [provided by RefSeq, Jul 2008]
Uniprot Description
FGF10: Plays an important role in the regulation of embryonic development, cell proliferation and cell differentiation. Required for normal branching morphogenesis. May play a role in wound healing. Interacts with FGFR1 and FGFR2. Interacts with FGFBP1. Belongs to the heparin-binding growth factors family.
Protein type: Secreted; Cytokine; Secreted, signal peptide; Cell development/differentiation; Motility/polarity/chemotaxis
Chromosomal Location of Human Ortholog: 5p13-p12
Cellular Component: extracellular matrix; extracellular space; cell surface; extracellular region; plasma membrane; nucleus
Molecular Function: heparin binding; protein binding; growth factor activity; type 2 fibroblast growth factor receptor binding; chemoattractant activity; fibroblast growth factor receptor binding
Biological Process: nerve growth factor receptor signaling pathway; salivary gland development; urothelial cell proliferation; activation of MAPK activity; somatic stem cell maintenance; positive regulation of epithelial cell proliferation involved in wound healing; positive regulation of transcription, DNA-dependent; muscle cell fate commitment; response to lipopolysaccharide; regulation of saliva secretion; embryonic pattern specification; G1/S-specific positive regulation of cyclin-dependent protein kinase activity; epithelial cell proliferation; positive regulation of fibroblast proliferation; positive chemotaxis; embryonic digestive tract morphogenesis; induction of an organ; mesonephros development; embryonic genitalia morphogenesis; spleen development; positive regulation of keratinocyte migration; positive regulation of DNA repair; fibroblast growth factor receptor signaling pathway; positive regulation of urothelial cell proliferation; positive regulation of peptidyl-tyrosine phosphorylation; branching morphogenesis of a tube; smooth muscle cell differentiation; positive regulation of transcription from RNA polymerase II promoter; determination of left/right symmetry; metanephros development; positive regulation of epithelial cell proliferation; wound healing; radial glial cell differentiation; positive regulation of mitotic cell cycle; positive regulation of vascular endothelial growth factor receptor signaling pathway; response to estradiol stimulus; induction of positive chemotaxis; negative regulation of cell proliferation; positive regulation of MAPKKK cascade; establishment of mitotic spindle orientation; tissue regeneration; positive regulation of lymphocyte proliferation; pancreas development; male genitalia morphogenesis; thyroid gland development; angiogenesis; lacrimal gland development; otic vesicle formation; female genitalia morphogenesis; positive regulation of Notch signaling pathway; epidermal growth factor receptor signaling pathway; hair follicle morphogenesis; phosphoinositide-mediated signaling; thymus development; keratinocyte proliferation; regulation of activin receptor signaling pathway; embryonic camera-type eye development; odontogenesis of dentine-containing teeth; limb bud formation; pituitary gland development; positive regulation of ATPase activity; actin cytoskeleton reorganization; white fat cell differentiation; insulin receptor signaling pathway; innate immune response; blood vessel remodeling; positive regulation of Ras protein signal transduction; positive regulation of DNA replication; regulation of smoothened signaling pathway
Disease: Lacrimoauriculodentodigital Syndrome; Aplasia Of Lacrimal And Salivary Glands
Research Articles on FGF-10
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Product Notes
The Bovine FGF-10 fgf10 (Catalog #AAA742003) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA742003 ELISA Kit recognizes Bovine FGF-10. It is sometimes possible for the material contained within the vial of "Fibroblast growth factor-10, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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