Typical Testing Data/Standard Curve (for reference only)
General fibrinogen total antigen assay ELISA Kit | FGA elisa kit
Rabbit fibrinogen total antigen assay ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: Rabbit fibrinogen will bind to the affinity purified capture antibody coated on the microtiter plate. After appropriate washing steps, biotin labeled polyclonal anti-rat fibrinogen primary antibody binds to the captured protein. Excess antibody is washed away and bound polyclonal antibody is reacted with streptavidin conjugated to horseradish peroxidase. TMB substrate is used for color development at 450nm. A standard calibration curve is prepared along with the samples to be measured using dilutions of rabbit fibrinogen. Color development is proportional to the concentration of fibrinogen in the samples.
Background: Fibrinogen is a soluble glycoprotein that circulates in the blood and is converted to insoluble fibrin by thrombin in the final step of the coagulation cascade [1]. Hepatic expression of fibrinogen increases two to four hundred fold during the acute phase response to infection or inflammation [2]. Elevated fibrinogen levels are correlated with cardiovascular disease [3] and atherosclerosis [4].
NCBI and Uniprot Product Information
NCBI Description
plays a role in blood coagulation; may be involved in liver regeneration [RGD, Feb 2006]
Uniprot Description
FGA: Fibrinogen has a double function: yielding monomers that polymerize into fibrin and acting as a cofactor in platelet aggregation
Protein type: Secreted; Secreted, signal peptide
Cellular Component: extracellular space; cell surface; rough endoplasmic reticulum; fibrinogen complex; cytoplasm; cell cortex; vesicle; external side of plasma membrane
Molecular Function: protein binding, bridging; cell adhesion molecule binding; structural molecule activity; receptor binding
Biological Process: protein polymerization; platelet activation; positive regulation of heterotypic cell-cell adhesion; cell-matrix adhesion; response to morphine; signal transduction; response to estradiol stimulus; response to genistein; cellular protein complex assembly; positive regulation of protein secretion; acute-phase response; positive regulation of vasoconstriction; response to cycloheximide; innate immune response; blood coagulation; response to calcium ion; positive regulation of exocytosis
Research Articles on FGA
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Product Notes
The General FGA fga (Catalog #AAA135501) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA135501 ELISA Kit recognizes General FGA. It is sometimes possible for the material contained within the vial of "fibrinogen total antigen assay, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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