Rabbit anti-Human, Mouse FUS Polyclonal Antibody | anti-FUS antibody

FUS, CT (FUS, TLS, RNA-binding protein FUS, 75kD DNA-pairing protein, Oncogene FUS, Oncogene TLS, POMp75, Translocated in liposarcoma protein) (FITC)

Cat. Num. AAA6300326

• Gene Names: FUS; TLS; ALS6; ETM4; FUS1; POMP75; HNRNPP2
• Reactivity: Human, Mouse
• Applications: Flow Cytometry, Functional Assay, Immunofluorescence, Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: FUS; Polyclonal Antibody; CT (FUS; TLS; RNA-binding protein FUS; 75kD DNA-pairing protein; Oncogene FUS; Oncogene TLS; POMp75; Translocated in liposarcoma protein) (FITC); Translocated in liposarcoma protein; anti-FUS antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-FUS antibody: Flow Cytometry (FC/FACS), FLISA, Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: FC/FACS: 1:10-50; FLISA: 1:1,000; IF: 1:10-50; IHC: 1:50-100; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: FUS antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 506-534 amino acids from the C-terminal region of human FUS.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(FUS Antibody (C-term) western blot analysis in mouse cerebellum tissue lysates (15ug/lane).This demonstrates the FUS antibody detected FUS protein (arrow).)

Immunohistochemistry (IHC)

(FUS antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the FUS antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(FUS Antibody (C-term) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Immunofluorescence (IF)

(Confocal immunofluorescent analysis of FUS Antibody (C-term) with MDA-MB231 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).)

Related Product Information for anti-FUS antibody

This gene encodes a multifunctional protein component of the heterogeneous nuclear ribonucleoprotein (hnRNP) complex. The hnRNP complex is involved in pre-mRNA splicing and the export of fully processed mRNA to the cytoplasm. This protein belongs to the FET family of RNA-binding proteins which have been implicated in cellular processes that include regulation of gene expression, maintenance of genomic integrity and mRNA/microRNA processing. Alternative splicing results in multiple transcript variants. Defects in this gene result in amyotrophic lateral sclerosis type 6.

Product Categories/Family for anti-FUS antibody

Antibodies; Binding Proteins

References

Kim, S.H., et al. J. Biol. Chem. 285(44):34097-34105(2010). Mackenzie, I.R., et al. Lancet Neurol 9(10):995-1007(2010). Yan, J., et al. Neurology 75(9):807-814(2010). Waibel, S., et al. Neurology 75(9):815-817(2010). Baumer, D., et al. Neurology 75(7):611-618(2010).

NCBI and Uniprot Product Information

• NCBI GI #: 283135201
• NCBI GeneID: 2521
• NCBI Accession #: NP_001164105.1
• NCBI GenBank Nucleotide #: NM_001170634.1
• UniProt Accession #: P35637; Q9H4A8
• Molecular Weight: 53426 MW
• NCBI Official Full Name: RNA-binding protein FUS isoform 2
• NCBI Official Synonym Full Names: FUS RNA binding protein
• NCBI Official Symbol: FUS
• NCBI Official Synonym Symbols: TLS; ALS6; ETM4; FUS1; POMP75; HNRNPP2
• NCBI Protein Information: RNA-binding protein FUS
• UniProt Protein Name: RNA-binding protein FUS
• Protein Family: Fusion protein
• UniProt Gene Name: FUS
• UniProt Synonym Gene Names: TLS
• UniProt Entry Name: FUS_HUMAN

NCBI Description

This gene encodes a multifunctional protein component of the heterogeneous nuclear ribonucleoprotein (hnRNP) complex. The hnRNP complex is involved in pre-mRNA splicing and the export of fully processed mRNA to the cytoplasm. This protein belongs to the FET family of RNA-binding proteins which have been implicated in cellular processes that include regulation of gene expression, maintenance of genomic integrity and mRNA/microRNA processing. Alternative splicing results in multiple transcript variants. Defects in this gene result in amyotrophic lateral sclerosis type 6. [provided by RefSeq, Sep 2009]

Uniprot Description

hnRNP P2: Binds both single-stranded and double-stranded DNA and promotes ATP-independent annealing of complementary single- stranded DNAs and D-loop formation in superhelical double-stranded DNA. May play a role in maintenance of genomic integrity. Component of nuclear riboprotein complexes. Interacts with ILF3, TDRD3 and SF1. Interacts through its C-terminus with SFRS13A. Interacts with OTUB1 and SARNP. Ubiquitous. Belongs to the RRM TET family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: RNA splicing; RNA-binding; Nuclear receptor co-regulator; Oncoprotein; DNA-binding

Chromosomal Location of Human Ortholog: 16p11.2

Cellular Component: nucleoplasm; cytoplasm; nucleus

Molecular Function: identical protein binding; protein binding; DNA binding; zinc ion binding; RNA binding; nucleotide binding

Biological Process: nuclear mRNA splicing, via spliceosome; RNA splicing; gene expression

Disease: Amyotrophic Lateral Sclerosis 6, With Or Without Frontotemporal Dementia; Tremor, Hereditary Essential, 4