Mouse Low affinity immunoglobulin gamma Fc region receptor III ELISA Kit | Fcgr3 elisa kit

Mouse Low affinity immunoglobulin gamma Fc region receptor III ELISA Kit

Cat. Num. AAA288193

• Gene Names: Fcgr3; CD16
• Reactivity: Mouse
• Synonyms: Low affinity immunoglobulin gamma Fc region receptor III; Mouse Low affinity immunoglobulin gamma Fc region receptor III ELISA Kit; Fcgr3 elisa kit

• Reactivity: Mouse
• Sequence Length: 250

• Assay Type: Sandwich
• Detection Range: 0.312-20 ng/ml
• Sensitivity: 0.19 ng/mL
• Intra-Assay CV: <=6.4%
• Inter-Assay CV: <=9.2%
• Recovery: 101%
• Preparation and Storage: For long term storage, please store the entire kit at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for Fcgr3 elisa kit

Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of target antigen concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for target antigen and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain target antigen, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 119877
• NCBI GeneID: 14131
• UniProt Accession #: P08508
• Molecular Weight: 30,036 Da
• NCBI Official Full Name: Low affinity immunoglobulin gamma Fc region receptor III
• NCBI Official Synonym Full Names: Fc receptor, IgG, low affinity III
• NCBI Official Symbol: Fcgr3
• NCBI Official Synonym Symbols: CD16
• NCBI Protein Information: low affinity immunoglobulin gamma Fc region receptor III
• UniProt Protein Name: Low affinity immunoglobulin gamma Fc region receptor III
• Protein Family: Low affinity immunoglobulin gamma Fc region receptor
• UniProt Gene Name: Fcgr3
• UniProt Synonym Gene Names: IgG Fc receptor III; FcRIII
• UniProt Entry Name: FCGR3_MOUSE

Uniprot Description

FCGR2A: low affinity receptor for the Fc region of IgGs. Binding to IgG initiates cellular responses against pathogens and soluble antigens. A type I membrane protein found on monocytes, neutrophils and platelets. SHP-1 associates with this protein to modulate signaling events in myeloid cells.

Protein type: Receptor, misc.; Cell surface; Membrane protein, integral

Cellular Component: external side of plasma membrane; integral to membrane; membrane; plasma membrane

Molecular Function: IgG binding; IgG receptor activity

Biological Process: antibody-dependent cellular cytotoxicity; antigen processing and presentation of exogenous peptide antigen via MHC class I; cell surface receptor linked signal transduction; mast cell activation; neutrophil chemotaxis; phagocytosis, engulfment; phagocytosis, recognition; positive regulation of phagocytosis; positive regulation of tumor necrosis factor biosynthetic process; positive regulation of type I hypersensitivity; positive regulation of type IIa hypersensitivity; positive regulation of type III hypersensitivity; regulation of immune response; serotonin secretion