Human Fc Fragment Of IgG Low Affinity IIIa Receptor (FcgR3A) ELISA Kit | FcgR3A elisa kit

Human Fc Fragment Of IgG Low Affinity IIIa Receptor (FcgR3A) ELISA Kit

Cat. Num. AAA453943

• Gene Names: FCGR3A; CD16; FCG3; CD16A; FCGR3; IGFR3; IMD20; FCR-10; FCRIII; FCGRIII; FCRIIIA
• Reactivity: Human
• Synonyms: Fc Fragment Of IgG Low Affinity IIIa Receptor (FcgR3A); Human Fc Fragment Of IgG Low Affinity IIIa Receptor (FcgR3A) ELISA Kit; CD16a; CD16-A; FCGR3-A; FCG3; FcgRIII; IGFR3; NA; Neutrophil Antigen; Low affinity immunoglobulin gamma Fc region receptor III-A; FcgR3A elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of FATP4. No significant cross-reactivity or interference between FATP4 and analogues was observed.
• Sequence Length: 359

• Samples: Human Serum, Plasma, Tissue Homogenates Or Other Biological Fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 0.156-10ng/mL
• Sensitivity: 0.068ng/mL.
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level FATP4 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level FATP4 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for FcgR3A elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of FATP4 in human serum, plasma, tissue homogenates or other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to FATP4. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to FATP4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain FATP4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of FATP4 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 1041817992
• NCBI GeneID: 2214
• NCBI Accession #: NP_000560.6
• NCBI GenBank Nucleotide #: NM_000569.7
• UniProt Accession #: P08637; Q53FJ0; Q53FL6; Q5EBR4; Q65ZM6; Q6PIJ0; A2N6W9
• Molecular Weight: 29,089 Da
• NCBI Official Full Name: low affinity immunoglobulin gamma Fc region receptor III-A isoform a
• NCBI Official Synonym Full Names: Fc fragment of IgG receptor IIIa
• NCBI Official Symbol: FCGR3A
• NCBI Official Synonym Symbols: CD16; FCG3; CD16A; FCGR3; IGFR3; IMD20; FCR-10; FCRIII; FCGRIII; FCRIIIA
• NCBI Protein Information: low affinity immunoglobulin gamma Fc region receptor III-A
• UniProt Protein Name: Low affinity immunoglobulin gamma Fc region receptor III-A
• Protein Family: Low affinity immunoglobulin gamma Fc region receptor
• UniProt Gene Name: FCGR3A
• UniProt Synonym Gene Names: CD16A; FCG3; FCGR3; IGFR3; Fc-gamma RIII; Fc-gamma RIIIa; FcRIII; FcRIIIa

NCBI Description

This gene encodes a receptor for the Fc portion of immunoglobulin G, and it is involved in the removal of antigen-antibody complexes from the circulation, as well as other other antibody-dependent responses. This gene (FCGR3A) is highly similar to another nearby gene (FCGR3B) located on chromosome 1. The receptor encoded by this gene is expressed on natural killer (NK) cells as an integral membrane glycoprotein anchored through a transmembrane peptide, whereas FCGR3B is expressed on polymorphonuclear neutrophils (PMN) where the receptor is anchored through a phosphatidylinositol (PI) linkage. Mutations in this gene have been linked to susceptibility to recurrent viral infections, susceptibility to systemic lupus erythematosus, and alloimmune neonatal neutropenia. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

Receptor for the Fc region of IgG. Binds complexed or aggregated IgG and also monomeric IgG. Mediates antibody-dependent cellular cytotoxicity (ADCC) and other antibody-dependent responses, such as phagocytosis.