Typical Testing Data/Standard Curve (for reference only)
Human Fc Fragment Of IgG Low Affinity IIIb Receptor (FcgR3B) ELISA Kit | FcgR3B elisa kit
Human Fc Fragment Of IgG Low Affinity IIIb Receptor (FcgR3B) ELISA Kit
Gene Names
FCGR3B; CD16; FCG3; CD16A; CD16b; FCGR3; FCGR3A; FCR-10; FCRIII; FCRIIIb
Reactivity
Human
Synonyms
Fc Fragment Of IgG Low Affinity IIIb Receptor (FcgR3B); Human Fc Fragment Of IgG Low Affinity IIIb Receptor (FcgR3B) ELISA Kit; CD16b; CD16-B; FCGR3-B; FCG3; FcgRIII; Low affinity immunoglobulin gamma Fc region receptor III-B; FcgR3B elisa kit
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of FcgR3B. No significant cross-reactivity or interference between FcgR3B and analogues was observed.
Sequence Length
233
Samples
Serum, plasma, tissue homogenates, cell lysates and other biological fluids
Assay Type
Quantitative Sandwich
Detection Range
0.312-20ng/mL
Sensitivity
<0.135ng/mL.
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level FcgR3B were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level FcgR3B were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
Preparation and Storage
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for FcgR3B elisa kit
Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of FcgR3B in human serum, plasma, tissue homogenates, cell lysates and other biological fluids.
Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to FcgR3B. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to FcgR3B. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain FcgR3B, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of FcgR3B in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to FcgR3B. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to FcgR3B. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain FcgR3B, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of FcgR3B in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
26,216 Da
NCBI Official Full Name
Fc fragment of IgG, low affinity IIIb, receptor (CD16b)
NCBI Official Synonym Full Names
Fc fragment of IgG receptor IIIb
NCBI Official Symbol
FCGR3B
NCBI Official Synonym Symbols
CD16; FCG3; CD16A; CD16b; FCGR3; FCGR3A; FCR-10; FCRIII; FCRIIIb
NCBI Protein Information
low affinity immunoglobulin gamma Fc region receptor III-B
UniProt Protein Name
Low affinity immunoglobulin gamma Fc region receptor III-B
Protein Family
UniProt Gene Name
FCGR3B
UniProt Synonym Gene Names
CD16B; FCG3; FCGR3; IGFR3; Fc-gamma RIII; Fc-gamma RIIIb; FcRIII; FcRIIIb
NCBI Description
The protein encoded by this gene is a low affinity receptor for the Fc region of gamma immunoglobulins (IgG). The encoded protein acts as a monomer and can bind either monomeric or aggregated IgG. This gene may function to capture immune complexes in the peripheral circulation. Several transcript variants encoding different isoforms have been found for this gene. A highly-similar gene encoding a related protein is also found on chromosome 1. [provided by RefSeq, Aug 2012]
Uniprot Description
Receptor for the Fc region of immunoglobulins gamma. Low affinity receptor. Binds complexed or aggregated IgG and also monomeric IgG. Contrary to III-A, is not capable to mediate antibody-dependent cytotoxicity and phagocytosis. May serve as a trap for immune complexes in the peripheral circulation which does not activate neutrophils.
Research Articles on FcgR3B
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Product Notes
The Human FcgR3B fcgr3b (Catalog #AAA2703102) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2703102 ELISA Kit recognizes Human FcgR3B. It is sometimes possible for the material contained within the vial of "Fc Fragment Of IgG Low Affinity IIIb Receptor (FcgR3B), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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