Sheep F-Box Protein 2 ELISA Kit | FBXO2 elisa kit

Sheep F-Box Protein 2 ELISA Kit

Cat. Num. AAA745669

• Gene Names: FBXO2; FBG1; FBX2; Fbs1; OCP1; NFB42
• Reactivity: Sheep
• Synonyms: F-Box Protein 2; Sheep F-Box Protein 2 ELISA Kit; FBXO2 elisa kit

• Reactivity: Sheep

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Sensitivity: 0.1 ng/mL.
• Intended Uses: This FBXO2 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Sheep FBXO2. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for FBXO2 elisa kit

Principle of the assay: FBXO2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-FBXO2 antibody and an FBXO2-HRP conjugate. The assay sample and buffer are incubated together with FBXO2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the FBXO2 concentration since FBXO2 from samples and FBXO2-HRP conjugate compete for the anti-FBXO2 antibody binding site. Since the number of sites is limited, as more sites are occupied by FBXO2 from the sample, fewer sites are left to bind FBXO2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The FBXO2 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for FBXO2 elisa kit

Epigenetics and Nuclear Signaling

NCBI and Uniprot Product Information

• NCBI GI #: 49457216
• NCBI GeneID: 26232
• UniProt Accession #: Q9UK22; Q5TGY0; Q6FGJ7; Q8TB29; Q9UKC6; B2R7K7
• Molecular Weight: 33,328 Da
• NCBI Official Full Name: FBXO2
• NCBI Official Synonym Full Names: F-box protein 2
• NCBI Official Symbol: FBXO2
• NCBI Official Synonym Symbols: FBG1; FBX2; Fbs1; OCP1; NFB42
• NCBI Protein Information: F-box only protein 2; F-box gene 1; organ of Corti protein 1
• UniProt Protein Name: F-box only protein 2
• Protein Family: F-box only protein
• UniProt Gene Name: FBXO2
• UniProt Synonym Gene Names: FBX2
• UniProt Entry Name: FBX2_HUMAN

NCBI Description

This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of the ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbxs class. This protein is highly similar to the rat NFB42 (neural F Box 42 kDa) protein which is enriched in the nervous system and may play a role in maintaining neurons in a postmitotic state. [provided by RefSeq, Jul 2008]

Uniprot Description

FBXO2: Substrate recognition component of a SCF (SKP1-CUL1-F- box protein) E3 ubiquitin-protein ligase complex that mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Involved in the endoplasmic reticulum-associated degradation pathway (ERAD) for misfolded lumenal proteins by recognizing and binding sugar chains on unfolded glycoproteins that are retrotranslocated into the cytosol and promoting their ubiquitination and subsequent degradation. Prevents formation of cytosolic aggregates of unfolded glycoproteins that have been retrotranslocated into the cytosol. Able to recognize and bind denatured glycoproteins, preferentially those of the high-mannose type.

Protein type: Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: 1p36.22

Cellular Component: endoplasmic reticulum; dendritic spine; SCF ubiquitin ligase complex; cytosol

Molecular Function: beta-amyloid binding; ubiquitin-protein ligase activity; glycoprotein binding; carbohydrate binding

Biological Process: negative regulation of cell proliferation; SCF-dependent proteasomal ubiquitin-dependent protein catabolic process; ER-associated protein catabolic process; regulation of protein ubiquitination; protein modification process; protein ubiquitination; glycoprotein catabolic process; proteolysis