Rat anti-Mouse Ly6G Monoclonal Antibody | anti-Ly6G antibody

Anti-Ms Ly6G Biotin

Cat. Num. AAA1801878

• Reactivity: Mouse
• Applications: Western Blot, Immunohistochemistry, Flow Cytometry, Functional Assay, Immunoprecipitation, Immunohistochemistry
• Purity: The purified antibody is conjugated with biotin-LC-NHS under optimum conditions. The reagent is free of unconjugated biotin.
• Synonyms: Ly6G; Monoclonal Antibody; Anti-Ms Ly6G Biotin; Gr1; Gr-1; Ly-6G; lymphocyte antigen 6 complex; locus G; anti-Ly6G antibody

• Host: Rat
• Reactivity: Mouse
• Clonality: Monoclonal
• Isotype: Rat IgG2b
• Clone Number: RB6-8C5
• Specificity: The rat monoclonal antibody RB6-8C5 detects Ly6G component of Gr-1 antigen, a commonly used surface marker of neutrophils.
• Purity/Purification: The purified antibody is conjugated with biotin-LC-NHS under optimum conditions. The reagent is free of unconjugated biotin.
• Form/Format: Stabilizing phosphate buffered saline (PBS) solution containing 15mM sodium azide
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-Ly6G antibody: Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Flow Cytometry (FC/FACS) (QC tested), Immunoprecipitation (IP), Immunohistochemistry-Frozen (IHC-F)
• Application Notes: FC/FACS: Recommended dilution: 2-5ug/ml
• Conjugation: Biotin
• Immunogen: Murine granulocytes
• Preparation and Storage: Store at 2-8 degree C. Do not freeze. Do not use after expiration date stamped on the label.

Flow Cytometry (FC/FACS)

(Flow cytometry surface staining pattern of murine peritoneal fluid cell suspension stained using anti-murine Ly6G (RB6-8C5) Biotin antibody (concentration in sample 1,0 ug/ml, Streptavidin APC).)

Flow Cytometry (FC/FACS)

(Separation of murine myeloid Ly6G positive cells stained using anti-Ly6G (RB-8C5) Biotin antibody (concentration in sample 1,0 ug/ml, Streptavidin APC, red-filled) from murine myeloid cells unstained by primary antibody (Streptavidin APC, black-dashed) in flow cytometry analysis.)

Related Product Information for anti-Ly6G antibody

Ly6G is a component of the myeloid differentiation antigen Gr-1, together with Ly6C. Ly6G is a good marker for detection of peripheral neutrophils. Expression of Gr-1 in bone marrow correlates with granulocyte differentiation and maturation. Physiological role of Ly6G remains still unclear. Its treatment with antibodies in vivo leads to neutropenia and has inhibitory effect on local immune responses.

References

*Fan J, Li Y, Levy RM, Fan JJ, Hackam DJ, Vodovotz Y, Yang H, Tracey KJ, Billiar TR, Wilson MA: Hemorrhagic shock induces NAD(P)H oxidase activation in neutrophils: role of HMGB1-TLR4 signaling. J Immunol. 2007 May 15;178(10):6573-80., URL: https://www.ncbi.nlm.nih.gov/pubmed/17475888ordin,*Koo GC, Gan YH: The innate interferon gamma response of BALB/c and C57BL/6 mice to in vitro Burkholderia pseudomallei infection. BMC Immunol. 2006 Aug 18;7:19., URL: https://www.ncbi.nlm.nih.gov/pubmed/16919160ordin,*dos Santos MS, Vaz Cardoso LP, Nascimento GR, Lino Rde S Jr, Dorta ML, de Oliveira MA, Ribeiro-Dias F: Leishmania major: recruitment of Gr-1+ cells into draining lymph nodes during infection is important for early IL-12 and IFN gamma production. Exp Parasitol. 2008 Jul;119(3):403-10., URL: https://www.ncbi.nlm.nih.gov/pubmed/18501355ordin,*Leendertse M, Willems RJ, Giebelen IA, Roelofs JJ, Bonten MJ, van der Poll T: Neutrophils are essential for rapid clearance of Enterococcus faecium in mice. Infect Immun. 2009 Jan;77(1):485-91., URL: https://www.ncbi.nlm.nih.gov/pubmed/19001080ordin,*Jung YW, Zindl CL, Lai JF, Weaver CT, Chaplin DD: MMP induced by Gr-1+ cells are crucial for recruitment of Th cells into the airways. Eur J Immunol. 2009 Aug;39(8):2281-92., URL: https://www.ncbi.nlm.nih.gov/pubmed/19593770ordin,*Hestdal K, Ruscetti FW, Ihle JN, Jacobsen SE, Dubois CM, Kopp WC, Longo DL, Keller JR: Characterization and regulation of RB6-8C5 antigen expression on murine bone marrow cells. J Immunol. 1991 Jul 1;147(1):22-8., URL: https://www.ncbi.nlm.nih.gov/pubmed/1711076ordina,*Fleming TJ, Fleming ML, Malek TR: Selective expression of Ly-6G on myeloid lineage cells in mouse bone marrow. RB6-8C5 mAb to granulocyte-differentiation antigen (Gr-1) detects members of the Ly-6 family. J Immunol. 1993 Sep 1;151(5):2399-408., URL: https://www.ncbi.nlm.nih.gov/pubmed/8360469ordina,*Stoppacciaro A, Melani C, Parenza M, Mastracchio A, Bassi C, Baroni C, Parmiani G, Colombo MP: Regression of an established tumor genetically modified to release granulocyte colony-stimulating factor requires granulocyte-T cell cooperation and T cell-produced interferon gamma. J Exp Med. 1993 Jul 1;178(1):151-61., URL: https://www.ncbi.nlm.nih.gov/pubmed/7686211ordina,*Brown CR, Blaho VA, Loiacono CM: Susceptibility to experimental Lyme arthritis correlates with KC and monocyte chemoattractant protein-1 production in joints and requires neutrophil recruitment via CXCR2. J Immunol. 2003 Jul 15;171(2):893-901., URL: https://www.ncbi.nlm.nih.gov/pubmed/12847259ordin,*Nagendra S, Schlueter AJ: Absence of cross-reactivity between murine Ly-6C and Ly-6G. Cytometry A. 2004 Apr;58(2):195-200., URL: https://www.ncbi.nlm.nih.gov/pubmed/15057973ordin

NCBI and Uniprot Product Information

• NCBI GeneID: 546644
• UniProt Accession #: A0A087WQF5
• Protein Family: Lymphocyte antigen