Human CD95 ELISA Kit | FAS elisa kit

Human CD95 ELISA Kit

Cat. Num. AAA8244614

• Gene Names: FAS; APT1; CD95; FAS1; APO-1; FASTM; ALPS1A; TNFRSF6
• Reactivity: Human
• Applications: ELISA
• Synonyms: CD95; Human CD95 ELISA Kit; APT1; FAS1; TNFRSF6; Tumor necrosis factor receptor superfamily member 6; Apo-1 antigen; Apoptosis-mediating surface antigen FAS; FASLG receptor; FAS elisa kit

• Reactivity: Human
• Specificity: The Human CD95 ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Human CD95 proteins within the range of 125 pg/ml - 8000 pg/ml.
• Sequence Length: 335

• Applicable Applications for FAS elisa kit: Sandwich ELISA (EIA)
• Samples: Cell Culture Supernatants, Serum, Plasma.
• Sensitivity: 62 pg/ml.
• Preparation and Storage: Shipped and store at 4 degree C for 6 months, store at -20 degree C for one year. Avoid freeze/thaw cycles.

Typical Testing Data/Standard Curve

Related Product Information for FAS elisa kit

Principle of the Assay||The Human CD95 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human CD95 in Cell Culture Supernatants, Serum, Plasma. This assay employs an antibody specific for Human CD95 coated on a 96-well plate. Standards and samples are pipetted into the wells and CD95 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human CD95 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CD95 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Background/Introduction: Fas, also known as APO-1, CD95 and TNFRSF6, is a member of the nerve growth factor (NGF)/tumour necrosis factor (TNF) receptor superfamily and mediates apoptosis. The nucleotide sequence of the cDNAs reveales that the molecule coding for the Fas antigen determinant is a 319 amino acid polypeptide with a single transmembrane domain. The extracellular domain is rich in cysteine residue, and shows a similarity to that of human tumor necrosis factor receptors, human nerve growth factor receptor, and human B cell antigen CD40. The APO-1 antigen as defined by the mouse monoclonal antibody anti-APO-1 is previously found to be expressed on the cell surface of activated human T and B lymphocytes and a variety of malignant human lymphoid cell lines. The APO-1 antigen is found to be a membrane glycoprotein of 48 kDa. Fas antigen is expressed and functional on papillary thyroid cancer cells and this may have potential therapeutic significance. Fas can play a role as an inducer of both neurite growth in vitro and accelerates recovery after nerve injury in vivo. The FAS and FASL triggered apoptosis pathway plays an important role in human carcinogenesis.

NCBI and Uniprot Product Information

• NCBI GI #: 119833
• NCBI GeneID: 355
• UniProt Accession #: P25445; Q14292; Q14293; Q14294; Q14295; Q16652; Q5T9P1; Q5T9P2; Q5T9P3; Q6SSE9; A9UJX4; B6VNV4
• Molecular Weight: 24,781 Da
• NCBI Official Full Name: Tumor necrosis factor receptor superfamily member 6
• NCBI Official Synonym Full Names: Fas cell surface death receptor
• NCBI Official Symbol: FAS
• NCBI Official Synonym Symbols: APT1; CD95; FAS1; APO-1; FASTM; ALPS1A; TNFRSF6
• NCBI Protein Information: tumor necrosis factor receptor superfamily member 6
• UniProt Protein Name: Tumor necrosis factor receptor superfamily member 6
• Protein Family: Tumor necrosis factor receptor superfamily
• UniProt Gene Name: FAS
• UniProt Synonym Gene Names: APT1; FAS1; TNFRSF6
• UniProt Entry Name: TNR6_HUMAN

NCBI Description

The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor contains a death domain. It has been shown to play a central role in the physiological regulation of programmed cell death, and has been implicated in the pathogenesis of various malignancies and diseases of the immune system. The interaction of this receptor with its ligand allows the formation of a death-inducing signaling complex that includes Fas-associated death domain protein (FADD), caspase 8, and caspase 10. The autoproteolytic processing of the caspases in the complex triggers a downstream caspase cascade, and leads to apoptosis. This receptor has been also shown to activate NF-kappaB, MAPK3/ERK1, and MAPK8/JNK, and is found to be involved in transducing the proliferating signals in normal diploid fibroblast and T cells. Several alternatively spliced transcript variants have been described, some of which are candidates for nonsense-mediated mRNA decay (NMD). The isoforms lacking the transmembrane domain may negatively regulate the apoptosis mediated by the full length isoform. [provided by RefSeq, Mar 2011]

Uniprot Description

FAS: Receptor for TNFSF6/FASLG. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death- inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. FAS- mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both. The secreted isoforms 2 to 6 block apoptosis (in vitro). Binds DAXX. Interacts with HIPK3. Part of a complex containing HIPK3 and FADD. Binds RIPK1 and FAIM2. Interacts with BRE and FEM1B. Interacts with FADD. Isoform 1 and isoform 6 are expressed at equal levels in resting peripheral blood mononuclear cells. After activation there is an increase in isoform 1 and decrease in the levels of isoform 6. 6 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Cell surface; Apoptosis; Receptor, cytokine

Chromosomal Location of Human Ortholog: 10q24.1

Cellular Component: apical plasma membrane; CD95 death-inducing signaling complex; cell soma; cell surface; cytoplasm; cytosol; external side of plasma membrane; extracellular space; integral to plasma membrane; lipid raft; neuron projection; nucleus; perinuclear region of cytoplasm; plasma membrane; sarcolemma; secretory granule

Molecular Function: identical protein binding; kinase binding; protease binding; protein binding; protein complex binding; receptor activity; signal transducer activity; tumor necrosis factor receptor activity

Biological Process: activated T cell apoptosis; aging; apoptosis; B cell mediated immunity; brain development; caspase activation; cell surface receptor linked signal transduction; chordate embryonic development; circadian rhythm; dendrite regeneration; immune response; immunoglobulin production; induction of apoptosis via death domain receptors; inflammatory cell apoptosis; inflammatory response; maternal process involved in pregnancy; negative regulation of apoptosis; negative regulation of B cell activation; negative regulation of caspase activity; negative thymic T cell selection; ovarian follicle atresia; ovulation cycle; positive regulation of apoptosis; positive regulation of MAPKKK cascade; positive regulation of protein homooligomerization; programmed cell death; protein complex assembly; protein homooligomerization; regulation of apoptosis; regulation of caspase activity; regulation of cell proliferation; regulation of gene expression; regulation of lymphocyte differentiation; regulation of myeloid cell differentiation; renal system process; response to cycloheximide; response to drug; response to glucocorticoid stimulus; response to lipopolysaccharide; response to peptide hormone stimulus; response to toxin; signal transduction; spermatogenesis; spleen development; telencephalon development; transformed cell apoptosis; tumor necrosis factor-mediated signaling pathway

Disease: Autoimmune Lymphoproliferative Syndrome