Western Blot (WB)
(All lanes : Anti-PHC3 Antibody (C-Term) at 1:2000 dilutionLane 1: Hela whole cell lysateLane 2: SH-SY5Y whole cell lysateLane 3: HepG2 whole cell lysateLane 4: U-2OS whole cell lysateLane 5: A549 whole cell lysateLane 6: MCF-7 whole cell lysateLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 106 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Rabbit anti-Human PHC3 Polyclonal Antibody | anti-PHC3 antibody
PHC3 Antibody (C-Term)
Western Blot (WB)
(All lanes : Anti-PHC3 Antibody (C-Term) at 1:2000 dilutionLane 1: Hela whole cell lysateLane 2: SH-SY5Y whole cell lysateLane 3: HepG2 whole cell lysateLane 4: U-2OS whole cell lysateLane 5: A549 whole cell lysateLane 6: MCF-7 whole cell lysateLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 106 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Western Blot (WB)
(All lanes : Anti-PHC3 Antibody (C-Term) at 1:2000 dilutionLane 1: Hela whole cell lysateLane 2: SH-SY5Y whole cell lysateLane 3: HepG2 whole cell lysateLane 4: U-2OS whole cell lysateLane 5: A549 whole cell lysateLane 6: MCF-7 whole cell lysateLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 106 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
NCBI and Uniprot Product Information
NCBI Description
PHC3: Component of a Polycomb group (PcG) multiprotein PRC1- like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. 7 isoforms of the human protein are produced by alternative splicing.
Protein type: Transcription regulation
Chromosomal Location of Human Ortholog: 3q26.2
Cellular Component: nucleoplasm; intracellular membrane-bound organelle; PcG protein complex; nucleus; actin cytoskeleton
Molecular Function: protein binding; DNA binding; zinc ion binding
Biological Process: protein sumoylation; cellular protein metabolic process; multicellular organismal development; post-translational protein modification
Uniprot Description
PHC3: Component of a Polycomb group (PcG) multiprotein PRC1- like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. 7 isoforms of the human protein are produced by alternative splicing.
Protein type: Transcription regulation
Chromosomal Location of Human Ortholog: 3q26.2
Cellular Component: actin cytoskeleton; intracellular membrane-bound organelle; nucleoplasm; nucleus; PcG protein complex
Molecular Function: DNA binding; protein binding; zinc ion binding
Biological Process: cellular protein metabolic process; multicellular organismal development; post-translational protein modification; protein sumoylation
Research Articles on PHC3
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Product Notes
The PHC3 phc3 (Catalog #AAA9215929) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PHC3 Antibody (C-Term) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PHC3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB ~~ 1:2000. Researchers should empirically determine the suitability of the PHC3 phc3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PHC3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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