Mouse Dentin matrix protein 4 ELISA Kit | FAM20C elisa kit

Mouse Dentin matrix protein 4 (FAM20C) ELISA Kit

Cat. Num. AAA284875

• Gene Names: Fam20c; DMP4; DMP-4; C76981; GEF-CK; mKIAA4081
• Reactivity: Mouse
• Synonyms: Dentin matrix protein 4; Mouse Dentin matrix protein 4 (FAM20C) ELISA Kit; DKFZp547C074; DMP4; RNS; dentin matrix protein 4; FAM20C elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of Mouse FAM20C. No significant cross-reactivity or interference between Mouse FAM20C and analogues was observed.
• Sequence Length: 579

• Samples: Serum, Plasma, Other biological fluids
• Assay Type: Sandwich
• Sample Volume: 1-200 uL
• Precision: Intra-assay Precision (Precision within an assay); Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.; Inter-assay Precision (Precision between assays); Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.; CV (%) = SD/meanX100; Intra-Assay: CV; Inter-Assay: CV
• Detection Wavelength: 450 nm
• Preparation and Storage: Store at 2-8 degree C.

Related Product Information for FAM20C elisa kit

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate FAM20C in samples. An antibody specific for FAM20C has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyFAM20C present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for FAM20C is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of FAM20C bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 167234439
• NCBI GeneID: 80752
• NCBI Accession #: NP_085042.2
• NCBI GenBank Nucleotide #: NM_030565.6
• UniProt Accession #: Q5MJS3; Q4FJP0; Q571A3; Q6PKA8; Q8JZP7
• Molecular Weight: 65,766 Da
• NCBI Official Full Name: extracellular serine/threonine protein kinase FAM20C isoform 1
• NCBI Official Synonym Full Names: family with sequence similarity 20, member C
• NCBI Official Symbol: Fam20c
• NCBI Official Synonym Symbols: DMP4; DMP-4; C76981; GEF-CK; mKIAA4081
• NCBI Protein Information: extracellular serine/threonine protein kinase FAM20C
• UniProt Protein Name: Extracellular serine/threonine protein kinase FAM20C
• Protein Family: Extracellular serine/threonine protein kinase
• UniProt Gene Name: Fam20c
• UniProt Synonym Gene Names: GEF-CK

Uniprot Description

FAM20C: an atypical serine protein kinase that localizes within the Golgi apparatus and is secreted. Distantly related to the Drosophila Golgi-localized kinase Four-jointed. Phosphorylates secretory proteins including the caseins and members of the small integrin-binding ligand, N-linked glycoprotein (SIBLING) protein family, which modulate biomineralization. Colocalizes with the Golgi resident protein GM130. Preferentially phosphorylates its targets within the S-x-E/pS motif. Requires Mn2+ as an alternative to Mg2+ as the activating cation. Defects in FAM20C are the cause of Raine syndrome (RNS). RNS is an autosomal recessive osteosclerotic bone dysplasia with neonatal lethal outcome. Clinical features include generalized osteosclerosis, craniofacial dysplasia and microcephaly. Two isoforms of the human protein are produced by alternative splicing.

Protein type: EC 2.7.11.1; Protein kinase, Ser/Thr (non-receptor); Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 5|5 G2

Cellular Component: cytoplasm; extracellular region; extracellular space; Golgi apparatus

Molecular Function: ATP binding; calcium ion binding; kinase activity; manganese ion binding; metal ion binding; nucleotide binding; protein serine/threonine kinase activity; transferase activity

Biological Process: ATP metabolic process; biomineral formation; phosphorylation; positive regulation of bone mineralization; positive regulation of osteoblast differentiation; protein amino acid phosphorylation; regulation of fibroblast growth factor receptor signaling pathway; regulation of phosphorus metabolic process; skeletal system development