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Western Blot (WB) (Western blot analysis of JPH1 in 293 cell lysate with JPH1 antibody at (A) 1 and (B) 2 μg/mL.)

Rabbit JPH1 Polyclonal Antibody | anti-JPH1 antibody

JPH1 Antibody

Gene Names
JPH1; JP1; JP-1
Reactivity
Human, Mouse, Rat
Applications
ELISA, Western Blot, Immunohistochemistry
Purity
JPH1 Antibody is affinity chromatography purified via peptide column.
Synonyms
JPH1; Polyclonal Antibody; JPH1 Antibody; JP1; JP-1; Junctophilin-1; Junctophilin type 1; junctophilin 1; anti-JPH1 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
JPH1 Antibody is affinity chromatography purified via peptide column.
Form/Format
Liquid
Concentration
1 mg/mL (varies by lot)
Sequence Length
658
Applicable Applications for anti-JPH1 antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
JPH1 antibody can be used for detection of JPH1 by Western blot at 1 - 2 mug/mL. Antibody can also be used for immunohistochemistry starting at 2.5 mug/mL.
Conjugate
Unconjugated
Immunogen
JPH1 antibody was raised against a 15 amino acid synthetic peptide near the carboxy terminus of human JPH1.
Buffer
JPH1 Antibody is supplied in PBS containing 0.02% sodium azide.
Preparation and Storage
JPH1 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Western Blot (WB)

(Western blot analysis of JPH1 in 293 cell lysate with JPH1 antibody at (A) 1 and (B) 2 μg/mL.)

Western Blot (WB) (Western blot analysis of JPH1 in 293 cell lysate with JPH1 antibody at (A) 1 and (B) 2 μg/mL.)

Immunohistochemistry (IHC)

(Immunohistochemistry of JPH1 in mouse skeletal muscle tissue with JPH1 antibody at 2.5 μg/mL.)

Immunohistochemistry (IHC) (Immunohistochemistry of JPH1 in mouse skeletal muscle tissue with JPH1 antibody at 2.5 μg/mL.)
Related Product Information for anti-JPH1 antibody
JPH1 Antibody: Junctional complexes between the plasma membrane (PM) and endoplasmic/sarcoplasmic reticulum (ER/SR) are a common feature of all excitable cell types and mediate cross talk between cell surface and intracellular ion channels. Junctophilins (JPs) are important components of the junctional complexes. JPs are composed of a carboxy-terminal hydrophobic segment spanning the ER/SR membrane and a remaining cytoplasmic domain that shows specific affinity for the PM. Four JPs have been identified as tissue-specific subtypes derived from different genes: JPH1 is expressed in skeletal muscle, JPH2 is detected throughout all muscle cell types, and JPH3 and JPH4 are predominantly expressed in the brain and contribute to the subsurface cistern formation in neurons. JPH1 is essential for stabilizing the T-tubule and SR membranes to form junctions and provide an environment for the assembly of receptors such as the ryanodine receptor type 1 (RyR1).

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
71,686 Da
NCBI Official Full Name
JPH1 protein, partial
NCBI Official Synonym Full Names
junctophilin 1
NCBI Official Symbol
JPH1
NCBI Official Synonym Symbols
JP1; JP-1
NCBI Protein Information
junctophilin-1; mitsugumin72; junctophilin type1; junctophilin type 1
UniProt Protein Name
Junctophilin-1
Protein Family
UniProt Gene Name
JPH1
UniProt Synonym Gene Names
JP1; JP-1
UniProt Entry Name
JPH1_HUMAN

NCBI Description

Junctional complexes between the plasma membrane and endoplasmic/sarcoplasmic reticulum are a common feature of all excitable cell types and mediate cross talk between cell surface and intracellular ion channels. The protein encoded by this gene is a component of junctional complexes and is composed of a C-terminal hydrophobic segment spanning the endoplasmic/sarcoplasmic reticulum membrane and a remaining cytoplasmic domain that shows specific affinity for the plasma membrane. This gene is a member of the junctophilin gene family. [provided by RefSeq, Jul 2008]

Uniprot Description

JPH1: Junctophilins contribute to the formation of junctional membrane complexes (JMCs) which link the plasma membrane with the endoplasmic or sarcoplasmic reticulum in excitable cells. Provides a structural foundation for functional cross-talk between the cell surface and intracellular calcium release channels. JPH1 contributes to the construction of the skeletal muscle triad by linking the t-tubule (transverse-tubule) and SR (sarcoplasmic reticulum) membranes. Belongs to the junctophilin family.

Protein type: Endoplasmic reticulum; Membrane protein, integral

Chromosomal Location of Human Ortholog: 8q21

Cellular Component: junctional membrane complex; integral to membrane; plasma membrane; Z disc

Molecular Function: structural constituent of muscle

Biological Process: muscle development

Research Articles on JPH1

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Product Notes

The JPH1 jph1 (Catalog #AAA150846) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The JPH1 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's JPH1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC). JPH1 antibody can be used for detection of JPH1 by Western blot at 1 - 2 mug/mL. Antibody can also be used for immunohistochemistry starting at 2.5 mug/mL. Researchers should empirically determine the suitability of the JPH1 jph1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "JPH1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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